| RP-HPLC法测定消炎利尿合剂中槲皮素的含量 |
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| 引用本文： | 李运景,卢荣枝,王小溪,邢洁,陈钧茂. RP-HPLC法测定消炎利尿合剂中槲皮素的含量[J]. 中国药房, 2007, 18(33): 2598-2600 |
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| 作者姓名： | 李运景 卢荣枝 王小溪 邢洁 陈钧茂 |
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| 作者单位： | 1. 广东中山市人民医院,中山市,528403
2. 华中科技大学同济医学院,武汉市,430030 |
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| 摘 要： | 目的:建立以反相高效液相色谱法测定消炎利尿合剂中槲皮素含量的方法。方法:色谱柱为Syltech-500C18(250mm×4.6mm,5μm),流动相为甲醇-0.2%磷酸(50∶50),流速为1.0mL.min-1,检测波长为360nm,柱温为25℃。结果:槲皮素检测浓度在19.2～96.0μg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9994);平均加样回收率为99.40%,RSD=2.31%(n=6)。结论:本方法专属性强、灵敏度高、干扰小、结果可靠,可用于消炎利尿合剂的质量控制。
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| 关 键 词： | 反相高效液相色谱法 槲皮素 消炎利尿合剂 含量测定 |
| 文章编号： | 1001-0408（2007）33-2598-03 |
| 收稿时间： | 2007-03-26 |
| 修稿时间： | 2007-06-22 |
Determination of the Content of Quercetin in Xiaoyanliniao Mixture by RP - HPLC |
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| LI Yunjing,LU Rongzhi,WANG Xiaoxi,XING Jie,CHEN Junmao. Determination of the Content of Quercetin in Xiaoyanliniao Mixture by RP - HPLC[J]. China Pharmacy, 2007, 18(33): 2598-2600 |
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| Authors: | LI Yunjing LU Rongzhi WANG Xiaoxi XING Jie CHEN Junmao |
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| Abstract: | OBJECTIVE:To establish RP-HPLC for the determination of the content of Quercetin in the Xiaoyanliniao mixture.METHODS:Samples were Separated on Syltech-500 C18 column(250mm×4.6mm,5μm).The mobile phase consisted of methanol-0.2% phosphoric acid(50∶50)at a flow rate of 1.0mL·min-1.The detection wavelength was 360nm,and the column temperature was 25℃.RESULTS:The linear range of Quercetin was from 19.2 to 96.0μg·mL-1(r=0.999 4).The average recovery was 99.40%(RSD=2.31%,n=6).CONCLUSION:The method was specific,sensitive and reliable in results yet with little interference,and it can be used as quality control for Xiaoyanliniao mixture. |
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| Keywords: | RP-HPLC Quercetin Xiaoyanliniao mixture Content determination |
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