早孕母胎界面固有模式识别受体NOD1/NOD2的表达

【目的】 明确早期妊娠母胎界面中固有模式识别受体NOD1/NOD2的表达情况?【方法】 分别用免疫组织化学法及Real-Time PCR法检测12例早孕期绒毛及蜕膜组织中NOD1/NOD2表达?【结果】免疫组织化学结果显示绒毛组织中NOD1/NOD2主要定位于绒毛滋养细胞胞质中,蜕膜组织也检测到NOD1/NOD2分子的表达?在mRNA水平上,绒毛组织中NOD1/NOD2的表达量高于蜕膜基质细胞(DSC),分别为P = 0.03和P = 0.009;DSC中NOD1的表达量高于NOD2,P = 0.029,差异具有显著性?绒毛组织中NOD2的表达高于NOD1,差异无统计学意义(P > 0.05)?【结论】 NOD1/NOD2在早孕期母胎界面中绒毛组织有表达,可能参与胚泡的着床?

Expression of Innate Pattern Recognition Receptors NOD1/NOD2 at Materno-Fetal Interface of Early Pregnancy

【Objective】 To characterize the expression of NOD1/NOD2 at materno-fetal interface. 【Methods】 NOD1 and NOD2 expression by villi and decidua were evaluated by immunohistochemistry and real-time PCR(n = 12). 【Results】 Immunohistochemistry result showed both NOD1 and NOD2 were expressed by villi and decidua at materno-fetal interface,and localized to cytoplasm of trophoblast cells and DSC. On the mRNA level, villi expressed more NOD1/NOD2 than decidual stromal cells(DSC,P = 0.03 and P = 0.009). DSC expressed more NOD1 than NOD2,P = 0.029. The differences were significant. Villi expressed more NOD2 than NOD1, but the difference was not significant (P > 0.05).【Conclusion】 Villi and DSC express NOD1/NOD2 at materno-fetal interface of early pregnancy. NOD1/NOD2 maybe participate in plantation of embryo.