慢性丙型肝炎患者HCV-RNA含量与CD4^+CD25^+调节性T细胞的相关性研究 |
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引用本文: | 陈志刚,左维泽,周迪,赵瑾.慢性丙型肝炎患者HCV-RNA含量与CD4^+CD25^+调节性T细胞的相关性研究[J].临床和实验医学杂志,2013,12(1):18-19. |
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作者姓名: | 陈志刚 左维泽 周迪 赵瑾 |
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作者单位: | 1. 石河子大学医学院第一附属医院医学实验室,新疆,石河子,832008 2. 石河子大学医学院第一附属医院感染科,新疆,石河子,832008 3. 石河子大学医学院病理学教研室,新疆,石河子,832002 |
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摘 要: | 目的探讨慢性丙型肝炎(CHC)患者外周血中CD4+CD25+调节性T细胞含量与HCV-RNA的相关性。方法用流式细胞仪检测80例CHC患者和30例健康对照者外周血CD4+CD25+Treg细胞表达,荧光定量PCR检测HCV-RNA含量。结果 CHC患者外周血中CD4+CD25+Treg细胞明显高于健康对照组(P〈0.01),且随HCV-RNA载量增高,患者外周血中CD4+CD25+Treg细胞的含量逐渐升高(P〈0.01)。CD4+CD25+Treg细胞与HCV-RNA取对数后呈正相关(r=0.588,P〈0.01),与ALT呈正相关(r=0.306,P〈0.01)。结论 CHC患者外周血中CD4+CD25+Treg细胞增高,且与HCV-RNA含量及ALT升高具有一致性。
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关 键 词: | 慢性丙型肝炎 HCV-RNA含量 CD4+CD25+调节性T细胞 |
Study on relationship between CD4+,CD25+ regulatory T cells and HCV-RNA load in peripheral blood of patients with chronic hepatitis C |
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Institution: | CHEN Zhi-gang1,ZUO Wei-ze2,ZHOU Di1,et al.1.Department of Clinical Laboratory;2.Department of Infection,The First Affiliated Hospital of Shihezi University Medical School,Shihezi Xinjiang 832008,China. |
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Abstract: | Objective To explore the amount of CD4 ^+ , CD25 ^+ regulatory T cells in peripheral blood of patients with chronic hepatitis C ( CHC ) and to study the relationship between these cells and HCV - RNA load. Methods The amount of CD4 ^+ , CD25 ^+ regulatory T cells in peripheral blood of 80 patients with chronic hepatitis C (CHC) and 30 healthy controls were detected by flow cytometry. The load of HCV - RNA was detected by fluorescent quantitative polymerase chain reaction ( FQ - PCR) assay. Results The amount of CD4 ^+ , CD25 ^+ regulatory T cells in peripheral blood of patients with chronic hepatitis C was significantly higher than those of control group ( P 〈0.01 ), and the amount of CD4 ^+ , CD25 ^+ regulatory T cells in peripheral blood of patients was gradually increased following the increase of HCV - RNA load ( P 〈 0.01 ). There was a positive correlation between amount of CD4 ^+ , CD25 ^+ regulatory T cells and HCV - RNA load after taking logarithm ( r =0. 588, P 〈0.01 ), and it was same as ALT ( r = 0. 306, P 〈 0.01 ). Conclusion The increase in amount of CD4 ^+ , CD25 ^+ regulatory T cells in patients with CHC is in accordance with copies of HCV DNA and increased level of ALT. |
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Keywords: | Chronic hepatitis C HCV - RNA content CD4 ^+ CD25 ^+ regulatory T cells |
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