The comparison of conventional and w.h.o. Methods for protein determination of chenopodium album allergenic extract

In present study different protein measurement methods are evaluated on the allergenic extract of Chenopodium album pollen, which was previously prepared in our laboratory and applied in skin prick testing in comparison with a commercial extract. The protein content of similar amounts of these two extracts which had caused similar skin reactions were measured with different protein assays such as protein nitrogen unit, Lowry procedure, ultra-violet absorption, and base catalyzed hydrolysis and ninhydrin (B.H.N.). The latter is recommended by World Health Organization (W.H.O.) in order to determine total protein of standard allergenic extracts. Our study indicated some differences between protein amounts measured by the various procedures. According to W.H.O. reports regarding the advantages of B.H.N. assay, due to good correlation with biological activity of allergenic extracts, we also found that this method can indicate the potency of extracts much more precisely.