Immediate postconditioning during reperfusion attenuates intestinal injury |
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Authors: | Ke-Xuan Liu Yun-Sheng Li Wen-Qi Huang Shu-Qing Chen Zhong-Xin Wang Jia-Xin Liu and Zhengyuan Xia |
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Institution: | (1) Department of Anesthesiology, The First Affiliated Hospital, Sun Yat-sen University, No. 58, Zhongshan 2th Road, 510080 Guangzhou, China;(2) Department of Gynecology and Obstetrics, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China;(3) Department of Anesthesiology, Research Centre of Heart, Brain, Hormone and Healthy Aging, University of Hong Kong, 102 Pokfulam Road, Hong Kong SAR, China |
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Abstract: | Objective To test the hypothesis that immediate but not delayed ischemic postconditioning (IPo) during reperfusion attenuates intestinal
injury, and that ischemic preconditioning (IPC) and IPo may confer synergy in intestinal protection.
Design and setting Prospective laboratory animal study with concurrent control.
Subjects Adult Sprague–Dawley rats.
Interventions Intestinal ischemia/reperfusion (II/R) injury in rats was produced by clamping superior mesenteric artery for 60 min followed
by 60 min reperfusion; IPC was elicited by 10 min ischemia and 10 min reperfusion before index ischemia; IPo was performed
by three cycles of 30 s reperfusion and 30 s ischemia initiated either immediately at the onset of reperfusion (IPo) or after
reperfusion for 3 min (delayed-IPo). Combination of IPC and IPo was performed by combining both protocols.
Measurements and main results Intestinal ischemia/reperfusion resulted in significant intestinal injury evidenced as significant increase in Chiu’s scores
and wet-to-dry intestine weight ratio accompanied with increases in plasma levels of tumor necrosis factor-α and interleukin-6,
as well as increases in the intestinal tissue lipid peroxidation product malonediadehyde and myeloperoxidase activity as compared
to control animals (all P < 0.05). All these changes were significantly attenuated either by IPC or IPo or their combination (P < 0.05), and not by delayed-IPo (P > 0.05). IPC and IPo showed synergistic protection compared with either protocol alone.
Conclusion Ischemic postconditioning reduces intestinal injury, in part, by inhibiting oxidative injury, neutrophils filtration and proinflammatory
response. The early period of reperfusion is critical to intestinal protection by IPo, and intestinal protection with IPo
can be enhanced by IPC.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
K-X. Liu and Z. Xia share senior authorship. |
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Keywords: | Postconditioning Preconditioning Intestinal reperfusion injury Oxidative injury |
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