γ-干扰素治疗免疫性大鼠肝纤维化的实验研究

目的观察γ-干扰素对猪血清诱导的大鼠免疫性肝纤维化的抑制作用.方法用猪血清腹腔注射复制免疫损伤性肝纤维化模型,9周后给大鼠每天肌肉注射γ-干扰素10万国际标准单位,共21天,以正常和模型组作为对照,第12周处死动物,测定体重、HA、LN、PCⅢ、Ⅳ-C并进行肝纤维化分级.结果γ-干扰素治疗组大鼠的肝纤维化生化指标HA、LN、PCⅢ、Ⅳ-C放免测定值较模型组明显下降(P＜0.01)而接近正常对照组(P＞0.05),肝纤维化的分级程度较模型组减轻(P＜0.01),大鼠体重较正常组和模型组稍有下降(P＞0.05).结论γ-干扰素能有效抑制猪血清诱导的大鼠免疫性肝纤维化,其作用表现为IFN-γ不仅能够抑制 胶原纤维进一步形成,更有强劲的促进胶原降解的作用.

Therapeutic effect of interferon gamma on rat with immune hepatic fibrosis

Objective: To observe the therapeutic effect of IFN-γ on rat with immune hepatic fibrosis induced by porcine serum injection. Methods: Porcine serum vas injected intraperitoneally to Wistar rats to produce the hepatic fibrosis model due to immunologic injury. After 9 weeks IFN-γ was used as therapeutic medicine, normal and model group as control. The animals were killed at 12th week to detect the levels of HA, LN, PC Ⅲ, Ⅳ-C and the grade of the pathologic fibrosis. Results: The levels of serum HA, LN, PC Ⅲ,ⅣV-C were lower in the IFN-y group than those in the model control group (P＜0.01), and similar to those in the normal control group (P＞0.05), the grade of liver fibrosis vas decreased apparently compared with the model control group (P＜0.01), but no significant difference in the rat′s weight among three groups. Conclu-sions: IFN-γ can suppress the progression of immune hepatic fibrosis of rats, through decreasing collagen synthesis and facilitating collagen degradation.