高效液相色谱-串联质谱法测定白内障患者应用加替沙星滴眼液后房水药物浓度

目的 定量分析白内障患者术前不同时间应用加替沙星(抗生素)滴眼液后房水中的药物浓度.方法 随机选取白内障患者36例,分为4组.各组患者均在术前3天内,于手术的眼局部应用加替沙星滴眼液,每日4次,每次1滴.手术当日,各组分别于术前15、30、60、120 min用加替沙星滴眼液1次;于术中取房水0.1 mL,用高效液相色谱-串联质谱法测量房水中的药物浓度.结果 4组房水中药物浓度分别为(0.15±0.29)、(0.33±0.22)、(0.31±0.19)、(0.74±0.32)μg·mL~(-1).结论 加替沙星滴眼液具有良好的角膜穿透性,局部应用能够在房水中获得有效的药物浓度;于术前120 min用药时,房水药物浓度最高.

Determination the aqueous humor concentration of gatifloxacin solution in cataract surgery patients by HPLC-MS/MS

Objective To Determine the aqueous concentration of gati-floxacin ophthalmic solution in the aqueous humor of patients undergoing cataract surgery. Methods Total 36 patients undergoing cataract surgery were divided into 4 groups randomly. All patients received topical gati-floxacin ophthalmic solution 4 doses every day for 3 days before cataract surgery. One drop of antibiotic was administered beginning 15 min prior to surgery for group 1, 30 min for group 2, 60 min for group 3, 120 min for group 4. Aqueous humor (0. 1 mL) was sampled via paracentesis. Catifloxacin concentrations were determined using validated high performance liquid chromatography/mass spectrometry procedures. Results Gatifloxacin concentrations in aqueous humor was (0. 15 ±0. 29) ,(0. 33 ±0.22), (0.31 ±0.19), (0.74 ±0.32) μg · mL~(-1) for 15, 30, 60, 120 min, respectively. Conclusion Gatifloxacin ophthalmic solution has extensive corneal penetration. The aqueous humor concentration is a-bove the minimum inhibitory concentration for important pathogens to a-chieve effective bacterial killing. Gatifloxacin concentration in aqueous humor was highest on 120 min.