肝移植后肝窦内皮细胞损伤的研究

目的 探讨肝移植后再灌注损伤和排斥反应时，肝窦内皮细胞的变化。方法 利用抗肝窦内皮细胞的单克隆抗体对大鼠肝移植后定期采取的肝组织标本染色。结果 对照组（LEW－LEW）于第4天在肝窦壁内可见ICAM－1的强染色，但是昆布氨酸、第Ⅷ因子相关抗原在实验基间均未见到。高度排斥反应组（ACI－LEW）移植后第1在开始发现ICAM－1的强染色。中度排斥反应组（BN－LEW）移植后第1天开始发现ICAM－1的强染色，移植后1个月开始肝窦壁出现第Ⅷ因子相关抗原。结论 说明慢性排斥反应引起的肝损伤，不仅是以肝动脉为中心的血管损伤，同时也损伤肝窦内皮细胞。

The injury endotheliocytes of hepatic sinusoid after liver transplantation

Objective To inquiry pathological changes of endotheliocytes ofhepatic sinusoid while reperfusion and rejection occurred after liver transplantation.Methods We stained liver specimen sampled termly with monoclonal antibodies to endotheliocytes of hepatic sinusoid in rat.Results ICAM-1 was stained markedly in hepatic sinusoid at 4th day,but we didn't find laminine and Ⅷ associated antigen during experiment.Clearly ICAM-1 stained was found in highly rejected group(ACI-LEW)from the first day after transplantation.In the medium group (BN-LEW),we detected that ICAM-1 was stained at 1st day after transplantation,and Ⅷ associated antigen was found in hepatic sinusoid.Conclusion The liver injury resulted from chronic rejection not only destroy hepatic artery but also injure hepatic sinusoid.