氧自由基及其清除剂在口腔粘膜炎性损害中的作用研究

目的 对氧自由基（ｏｘｙｇｅｎ ｆｒｅｅ ｒａｄｉｃａｌｓ，ＯＦＲｓ）及其清除剂超氧化物歧化酶（ｓｕｐｅｒｏｘｉｄｅ ｄｉｓ－ｍｕｔａｓｅ，ＳＯＤ）在口腔粘膜炎性损害中的作用机制进行系统研究。方法 ①建立ＯＦＲｓ致口腔粘膜急性炎症的动物模型，分别采用酶组织化学染色、组合导数分光光度法研究该损害中辅酶Ⅱ黄递酶等酶活性以及氨基己糖、氨基葡萄糖水平的变化；②分别采用电子顺磁共振、放射免疫技术研究外源性Ｓ

The roles of oxygen free radicals and superoxide dismutase in the inflammatory injury in animal oral mucosa

OBJECTIVE: To study the roles of oxygen free radicals (OFRs) and superoxide dismutase (SOD) in the inflammatory injury in animal oral mucosa. METHODS: 1. The animal model was established in which an acute oral mucosal inflammation was induced by OFRs. Enzyme histological methods and biochemical methods were used respectively to investigate the activities of some important enzymes such as nicotinamide adenine dinucleotide phosphate-diaphorase and the amount of aminohexoses in the lesions; 2. Electron spin resonance and radioimmunoassay were ultilize respectively to study the effects of exogenous SOD on the amount of OFRs and prostaglandin E2(PGE2) in the lesions. RESULTS: 1. The activities of the enzymes in the oral lesions decreased and the amount of aminohexoses increased significantly; 2. Local administrating SOD could reduce the amount of OFRs and PGE2 in the lesions sufficiently. CONCLUSION: OFRs induced the inflammatory lesion by injuring some important enzymes and connective tissues in the oral mucosa. Local administrating SOD could scavenge OFRs and PGE2 in the oral mucosa sufficiently and reduce the degree of OFRs--induced oral mucosal inflammatory injury.