| 枸杞多糖体外对正常及衰老小鼠免疫细胞功能的作用及作用机理的初步研究 |
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| 引用本文： | 齐春会,张永祥,陈保文,顾国明.枸杞多糖体外对正常及衰老小鼠免疫细胞功能的作用及作用机理的初步研究[J].中国免疫学杂志,1999,15(9):2. |
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| 作者姓名： | 齐春会 张永祥 陈保文 顾国明 |
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| 作者单位： | 军事医学科学院毒物药物研究所,北京100850 |
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| 基金项目： | 国家自然科学基金青年基金 |
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| 摘 要： | 目的：探讨枸杞多糖（ＬＢＰ） 对免疫细胞功能的直接作用及其机理。方法：应用淋巴细胞增殖、溶血空斑和Ｆｕｒａ２ＡＭ 双波长荧光测定等方法。结果：ＬＢＰ体外应用对正常小鼠及快速老化模型ＳＡＭＰ８ 和抗快速老化模型ＳＡＭＲ１ 脾细胞增殖反应具有直接促增殖作用，明显增加正常小鼠脾抗体形成细胞（ＰＦＣ） 数目，迅速诱导脾细胞和腹腔巨噬细胞内［Ｃａ２ ＋ ］ｉ 的增加，并有一定剂量依赖性关系。结论：枸杞多糖对免疫活性细胞的功能有直接促进作用，这可能与其增加细胞［Ｃａ２ ＋ ］ｉ 有关。
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| 关 键 词： | 枸杞多糖 淋巴细胞增殖反应 抗体形成细胞 游离Ca~(2 ) Fura-2-AM |
| 修稿时间： | 1998-04-27 |
Effects of lycium barbarum polysaccharides on the splenocyte immune function of normal and aged model mouse and it's possible mechanism in vitro |
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| QI Chun Hui,ZHANG Yong Xiang,CHEN Bao Wen et al.Effects of lycium barbarum polysaccharides on the splenocyte immune function of normal and aged model mouse and it's possible mechanism in vitro[J].Chinese Journal of Immunology,1999,15(9):2. |
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| Authors: | QI Chun Hui ZHANG Yong Xiang CHEN Bao Wen |
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| Institution: | QI Chun Hui,ZHANG Yong Xiang,CHEN Bao Wen et al.Institute of Pharmacology and Toxicology,Academy of Military Medical Science,Beijing 100850 |
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| Abstract: | Objective:To explore the action of immuno modulation of lycium barbarum polysaccharides(LBP) on mouse splenocyte and it's possible mechanism in vitro Methods: 3H TdR incorporation plaque forming cell(PFC) assays and Fura 2/AM fluorescence double wave measurement Results: LBP not only enhanced splenocyte proliferation of LACA,senescence accelerated mouse(SAMP8)and antisenescence accelerated mouse(SAMR1)directly,but also increased the number of PFC of LACA mouse splenocytes Furthermore,the concentration of free Ca 2 in splenocytes and macrophages was increased rapidly with dose depend manner induced by LBP Conclusion :LPB possesses direct immunopromoting effect on immune cell function and this effect maybe related with increasing concentration of free Ca 2 in cytoplasm,Ca 2 ] i |
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| Keywords: | Lycium barbarum polysaccharides(LBP) Antibody production response(PFC) Splenocyte proliferation response Concentration of free Ca 2 |
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