| 新型抗狂犬病病毒核蛋白抗体进行RFFIT检测效果评估 |
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| 引用本文： | 吕新军,马学军.新型抗狂犬病病毒核蛋白抗体进行RFFIT检测效果评估[J].国际检验医学杂志,2011,32(7):721-722,725. |
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| 作者姓名： | 吕新军 马学军 |
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| 作者单位： | 中国疾病预防控制中心病毒病预防控制所,北京,102206 |
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| 基金项目： | 十一五科技重大专项-传染病检测技术研究-传染病病原体诊断和组合检测技术 |
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| 摘 要： | 目的评价自主研发的荧光标记抗狂犬病病毒核蛋白单克隆抗体用于快速荧光灶抑制试验(RFFIT)的效果。方法将狂犬病病毒核蛋白的一段线性表位肽与KLH耦联后免疫Balb/c小鼠制备的高效价单克隆抗体抗-RVNP-Mcl,经浓缩、纯化、异硫氰酸荧光素(FITC)标记用于RFFIT检测,以Millipore公司DFA5100为对照试剂,对200例人血清进行平行RFFIT检测,将结果进行统计学分析。结果两种抗体检测结果的线性相关分析显示相关系数(r)=0.980;两种抗体检测定性结果一致率为96.83%,卡方检验显示两种抗体检测定性结果差异无统计学意义(χ2=0.098,P>0.05),发生定性结果不一致的6例(3.00%)血清两种抗体检测结果均小于1 IU/mL;68例两种抗体检测结果均小于或等于1 IU/mL的血清样品中有6例(8.82%)发生结果定性不一致,其余样品均未发生不一致的情况。结论使用荧光标记抗-RVNP-Mcl与DFA5100进行RFFIT检测时结果高度一致,荧光标记抗-RVNP-Mc1可供RFFIT检测使用。
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| 关 键 词： | 狂犬病病毒 核蛋白类 抗体 快速荧光灶抑制试验 |
Evaluation on the effect of new type of antibody against the nucleoprotein of rabies virus used for RFFIT |
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| Lv Xinjun,Ma Xuejun.Evaluation on the effect of new type of antibody against the nucleoprotein of rabies virus used for RFFIT[J].International Journal of Laboratory Medicine,2011,32(7):721-722,725. |
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| Authors: | Lv Xinjun Ma Xuejun |
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| Institution: | Lv Xinjun,Ma Xuejun(Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China) |
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| Abstract: | Objective To evaluate the effect of fluorescein isothiocyanate(FITC)labeled monoclonal antibody against the nucleoprotein of rabies virus used for rapid fluorescent focus inhibition test(RFFIT).Methods Anti-RVNP-Mc1,one strain of monoclonal antibody against the nucleoprotein of rabies virus was prepared by immunizing Balb/c mice with a piece of linear epitope peptide on the nucleoprotein of rabies virus linked with KLH molecular.Anti-RVNP-Mc1 was concentrated,purified and labeled by FITC.FITC-Anti-RVNP-Mcl ... |
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| Keywords: | rabies virus nucleoproteins antibodies rapid fluorescent focus inhibition test |
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