罗格列酮对局灶性脑缺血再灌注大鼠的保护作用

目的 研究不同剂量罗格列酮(rosiglitazone,RSG)对局灶性脑缺血再灌注大鼠的保护作用.方法 40只雄性Sprague-Dawley 大鼠随机分为假手术组、对照组、小剂量RSG组1mg/(kg·d)]、中剂量RSG组2 mg/(kg·d)]和大剂量RSG组4 mg/(kg·d)],每组8只.线栓法制备...

Protective effect of rosiglitazone against focal cerebral ischemia-reperfusion injury in rats

Objective To study the protective effect of different doses of rosiglitazone (RSG)against focal cerebral ischemia-reperfusion injury in rats．Methods Forty male sprague Dawley rats were randomly divided into the following groups：sham-operation，control，lowdose RSG(1 mg/kg·d)，moderate-dose(2 mg/kg·d)，high-dose(4 mg/kg·d)goups(n=8 in each grow)．A rat model of middle cerebral artery occlusion for 2 hours and reperfusion for 22 hours were induced by the intraluminal suture method．An automatie biochemical analyzer was used to detect the blood glucose and lipid levels．A solid phase sandwich enzyme-linked immunosorbent assay was used to determine serum insulin levels，and the insulin sensitivity indexes were calculated．The effect of rosiglitazone on infarct volume and behavioristics was observed．Results Compared with the control group，the infarct volume was significantly reduced，and the neurological function scores were improved significantly in each RSG group(P<0．05 or P<0．01)．The insulin sensitivity was significantly increased(P<0．05 or P<0．01)，and hyperglycemia was reduced significantly after cerebral ischemia(P<0．05)and showed a dose-dependent manner.Conclusions RSG has obvious neuroprotective effect against cerebral ischemia／repeffusion injury in rats．Its mechanism may be associated with the increase of insulin sensitivity．