100Hz电刺激肩胛间区对家兔胆囊痛及其脊髓后角磷酸化细胞外信号调节蛋白激酶表达的影响

目的 探讨100 Hz电刺激肩胛间区对家兔胆囊痛及脊髓后角磷酸化细胞外信号调节蛋白激酶(phosphorylate extracellular signal-regulated kinases,pERK1/2)表达的影响.方法 家兔采用随机数字表法随机分为5组:对照组、生理盐水组、甲醛溶液组、肩胛间区电刺激组和非肩胛间...

The effects of 100 Hz electrical stimulation at interscapular region on cholecystalgia and the expression of pERK1/2 in spinal posterior horn of rabbits

Objective To investigate the effects of 100 Hz electrical stimulation at the interscapular region on rabbit's cholecystalgia and the expression of pERK1/2 in spinal posterior horn.Methods Rabbits were randomly divided into five groups:control group,0.9%NS group,formalin group,stimulating interscapular group and non-stimulating interscapular group,six rabbits in each group. By using immunohistochemistry,physiological function test and Western blot technique,the variation of RR,HR and the expression of pERK1/2 in spinal posterior horn of rabbits with cholecystalgia were measured when 100Hz electrical stimulation was applied at the interscapular region of rabbits.Results The RR and HR in stimulating interscapular group were significantly decreased,and there was a 54% reduction on the pERK1/2 expression in spinal posterior horn compared with formalin group (P＜0.01). Conclusion 100 Hz electrical stimulation at interscapular region can partly alleviate the changes in physiological parameters and inhibit the activation of ERK1/2 in spinal posterior horn of rabbits with cholestagia.