隐球菌性脑膜炎及病毒性脑炎患者脑脊液IL-6、IL-10和TNF-α含量检测及其临床意义

目的:探讨隐球菌性脑膜炎及病毒性脑炎患者脑脊液中白细胞介素-6(IL-6)、IL-10和肿瘤坏死因子-α(TNF-α)含量的变化及临床意义.方法:应用Luminex液相芯片技术对隐球菌性脑膜炎患者18例(隐脑组),病毒性脑炎患者20例(病脑组)及非中枢神经系统感染患者15例(对照组)脑脊液中IL-6、IL-10和TNF...

Determination of Interleukin-6, Interleukin-10, and Tumor Necrosis Factor-alpha in Cerebrospinal Fluid of Patients with Cryptococcal Meningitis and Viral Encephalitis

Objective: To investigate the concentrations of interleukin-6(IL-6),IL-10 and tumor necrosis factor-α(TNF-α) in cerebrospinal fluid(CSF) of patients with cryptococcal meningitis or viral encephalitis and to analyze the clinical implication of the cases.Methods: Eighteen patients with cryptococcal meningitis(CM group),twenty with viral encephalitis(VE group) and fifteen control volunteers without any central nervous system infection(control group) were enrolled in the study.CSF samples were obtained at admission and the concentrations of IL-6,IL-10 and TNF-α were identified using the Luminex xMAP fluid microsphere-based array system.Results: The concentrations of IL-6 and TNF-α in CM group were significantly higher than those in VE(P<0.01) and control groups(P<0.05).The concentration of IL-10 was higher in CM group than that in the control group(P<0.001).In addition,the concentrations of IL-6,IL-10 and TNF-α were significantly increased in VE group compared with those in the control group(P<0.001;P<0.001;P<0.05).Correlation analysis showed that IL-6 was positively related to IL-10 in CM group(r=0.50,P<0.05).Conclusion: IL-6,IL-10 and TNF-α may play important roles in pathophysiological process of cryptococcal meningitis and viral encephalitis.More studies are needed to illustrate the association of prognosis and the changes in the cytokines.