坎地沙坦对肝纤维化大鼠Ang-(1-7)的影响

目的研究血管紧张素Ⅱ1型受体拮抗剂坎地沙坦抗大鼠肝纤维化的疗效及对血管紧张素1-7[Ang-(1-7)]的影响。方法制备四氯化碳诱导大鼠肝纤维化模型,同时应用坎地沙坦灌胃,共8周。肝组织进行常规HE与Masson三色染色,并测定门脉压及血清肝功能,血浆中Ang-(1-7)应用酶联免疫方法检测。结果与模型组大鼠比较,坎地沙坦可改善肝纤维化程度及降低门脉压,血浆Ang-(1-7)水平增加。结论坎地沙坦抗肝纤维化可能与升高Ang-(1-7)有关。

Effects of candesartan on Ang-(1-7) in experimental hepatic fibrosis in rats

Objective To explore the protective effect of angiotensin Ⅱtype 1(AT1) receptor blocker candesartan in hepatic fibrosis induced by carbon tetrachloride(CCL4) in rats and effects of candesartan on Ang-(1-7).Methods Except rats in control group,all were given intraperitoneal injections of 40% CCL4,and from the first day of the intraperitoneal injection,rats in treatment groups were given candesartan for 8 weeks by gastric gavage.Liver tissue and blood samples of all rats were examined at the end of 8 weeks.Histopathological study of liver tissue was done with hematoxylin-eosin(HE) and Masson trichrome staining.all rats were examined portal vein pressure.Blood samples were taken for detecting alanine aminotransferase(ALT) and the levels of Ang-(1-7)evaluated by ELASA.Results Compared with the health controls,candesartan significantly attenuated the degree of hepatic fibrosis and decreased portal vein pressure and plasma Ang-(1-7)levels increased.Conclusion Candesartan can retard the progression of hepatic fibrosis and exerts its antifibrogenic effect by adding Ang-(1-7)levels.