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| 中药复方海康灵含药血清对过氧化氢诱导的神经细胞凋亡的影响 | |
| 引用本文： | 孙向红,孙伟,李静,耿美玉.中药复方海康灵含药血清对过氧化氢诱导的神经细胞凋亡的影响[J].中国药学杂志,2008,43(23):1781-1784. |
| 作者姓名： | 孙向红 孙伟 李静 耿美玉 |
| 作者单位： | 1. 青岛大学医学院附属医院,山东青岛266003;中国海洋大学医药学院,山东青岛,266003 2. 青岛大学医学院附属医院,山东青岛,266003 3. 中国海洋大学医药学院,山东青岛,266003 |
| 摘 要： | 目的观察海康灵对SH-SY5Y神经细胞凋亡、细胞内线粒体膜电位和钙离子的影响。方法采用血清药理学方法,用流式细胞仪观察海康灵对过氧化氢(H2O2)损伤的SH-SY5Y神经细胞凋亡、细胞线粒体膜电位和钙离子的影响。结果与模型组比较,海康灵含药血清能明显抑制H2O2引起的SH-SY5Y神经细胞的凋亡,稳定线粒体膜电位,抑制钙离子内流。结论以上结果提示,海康灵可通过抑制胞浆钙离子升高,进而稳定线粒体膜电位,从而发挥其抑制神经细胞凋亡的作用。 |
| 关 键 词： | 海康灵 过氧化氢 凋亡 线粒体膜电位 钙离子 |
| 收稿时间： | 2007-12-21; |
Effects of Haikangling in Serum on Cultured Neuroblastoma Apoptosis Induced by Hydrogen Peroxide |
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| SUN Xiang-hong,SUN Wei,LI Jing,GENG Mei-yu.Effects of Haikangling in Serum on Cultured Neuroblastoma Apoptosis Induced by Hydrogen Peroxide[J].Chinese Pharmaceutical Journal,2008,43(23):1781-1784. | |
| Authors: | SUN Xiang-hong SUN Wei LI Jing GENG Mei-yu |
| Institution: | 1. Hospital Affiliated to Medical College，Qingdao University，Qingdao 266003，China； 2. School of Medicine and Pharmacy，Ocean University of China，Qingdao 266003，China |
| Abstract: | OBJECTIVE To observe the effect of Haikangling in serum on apoptosis,mitochondria membrane potential and Ca~(2 ) of neuroblastoma SH-SY5Y cells.METHODS The effects of Haikangling in serum on apoptosis,mitochondria membrane potential and Ca~(2 ) of SH-SY5Y cells impaired by hydogen peroxide(H_2O_2)cultured in vitro were measured by flow cytometry.RESULTS Compared with the model group,Haikangling obviously restrained the apoptosis,Ca~(2 ) inflow,and stabilized the mitochondria membrane potential of SH-SY5Y cells.CONCLUSION Anti-dementia effects of Haikangling were related to restraining the apoptosis,stabilizing mitochondria membrane potential and restraining Ca2 inflow of neurocyte. |
| Keywords: | Haikangling H_2O_2 apoptosis mitochondria membrane potential Ca~(2 ) |
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