| 低温冷冻对皮肤高分子量角蛋白表达的影响 |
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| 引用本文： | 蔡宏,董宁,姚咏明,李绍然,贾晓明.低温冷冻对皮肤高分子量角蛋白表达的影响[J].军医进修学院学报,2011,32(12):1271-1273. |
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| 作者姓名： | 蔡宏 董宁 姚咏明 李绍然 贾晓明 |
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| 作者单位： | 1. 空军总医院皮肤科,北京,100036
2. 解放军总医院第一附属医院,北京,100037 |
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| 摘 要： | 目的 研究皮肤低温储存后表皮层高分子量角蛋白的变化,从而分析皮肤组织细胞骨架的低温生物学效应.方法 将新鲜成人皮肤分为新鲜组、4℃组、-20℃组、-80C组和-196℃组,于冻存后第14天复温,运用免疫组织化学染色和电镜观察角蛋白的改变,并用图像分析仪测定表皮组织中的含量变化.结果 随着保存温度的降低,4℃、-20℃、-80℃组的角蛋白均表达下降,而-196℃组与新鲜皮肤组表达相似.结论 皮肤组织的低温损伤与细胞骨架结构密切相关,角蛋白在该低温生物学机制中发挥着重要作用.
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| 关 键 词： | 皮肤 高分子量角蛋白 低温 |
Effect of cryopreservation on expression of high molecular weight epidermal keratin |
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| CAI Hong
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DONG Ning
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YAO Yong-ming
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LI Shao-ran
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JIA Xiao-ming.Effect of cryopreservation on expression of high molecular weight epidermal keratin[J].Academic Journal of Pla Postgraduate Medical School,2011,32(12):1271-1273. |
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| Authors: | CAI Hong DONG Ning YAO Yong-ming LI Shao-ran JIA Xiao-ming |
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| Institution: | 1Department of Dermatology,General Hospital of Air Force,Beijing 100036,China;2First Affiliated Hospital,Chinese PLA General Hospital,Beijing 100037,China |
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| Abstract: | Objective To study the changes in cryo-preserved high molecular weight epidermal keratin and analyze the effect of cryobiology on epidermal cytoskeleton.Methods Adult human skin samples were divided into fresh group,4℃ group,-20℃ group,-80℃ group and-196℃ group.Changes in epidermal keratin were observed under an electron microscope with immunohistochemical staining 14 days after cryopreservation.Keratin level in skin tissue was analyzed with an image analyzer.Results The expression level of keratin decreased with the decreasing preservation temperature in 4℃ group,-20℃ group,and-80℃ group,and was similar in-196℃ group and fresh group.Conclusion Hypothermia injury of skin is closely correlated with cytoskeleton,and keratin plays an important role in the mechanism of cryobiology. |
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| Keywords: | skin high molecular weight hypothermia |
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