| 人参提取物对内毒素所致小鼠脾细胞损伤的保护作用 |
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| 引用本文： | 王兴翠,尚宏伟,仇文颖,魏育林,李宝红,孙海梅,郭崇洁. 人参提取物对内毒素所致小鼠脾细胞损伤的保护作用[J]. 解剖学报, 2003, 34(3): 312-316 |
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| 作者姓名： | 王兴翠 尚宏伟 仇文颖 魏育林 李宝红 孙海梅 郭崇洁 |
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| 作者单位： | 1. 首都医科大学组织学胚胎学教研室,北京,100054
2. 中日友好医院临床研究所,北京,100029 |
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| 摘 要： | 目的 观察人参提取物在内毒素脂多糖(LPS)所致的SIRS／MODS中对脾细胞的作用。方法 通过HE、TUNEL染色和透射电镜比较注射人参提取物(GS)后再注射LPS时的脾脏形态学及凋亡情况，并用免疫组织化学的方法检测Bcl-2，Fas，FasL的表达。结果 随LPS作用时间的延长，脾损伤逐渐加重。动脉周围淋巴鞘细胞碎片及TUNEL阳性细胞增多，电镜观察可见到凋亡的淋巴细胞。而人参组细胞损伤明显减轻。Bcl-2的表达随着LPS作用时间的延长而减少，Fas，FasL则增强。人参提取物主要阻止Bcl-2表达减少。结论 人参提取物对LPS脾细胞损伤有保护作用，其作用机制与增强Bcl-2表达有关。
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| 关 键 词： | 人参提取物 小鼠 脾细胞损伤 保护作用 内毒素血症 中药 |
PROTECT EFFECT OF GINSENG ABSTRACT ON MOUSE SPLECYTOES INJURY INDUCED BY LPS |
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| WANG Xing cui ,SHANG Hong wei ,QIU Wen ying ,WEI Yu lin ,LI Bao hong ,SUN Hai mei ,GUO Chong jie. PROTECT EFFECT OF GINSENG ABSTRACT ON MOUSE SPLECYTOES INJURY INDUCED BY LPS[J]. Acta Anatomica Sinica, 2003, 34(3): 312-316 |
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| Authors: | WANG Xing cui SHANG Hong wei QIU Wen ying WEI Yu lin LI Bao hong SUN Hai mei GUO Chong jie |
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| Affiliation: | WANG Xing cui 1,SHANG Hong wei 1,QIU Wen ying 1,WEI Yu lin 2,LI Bao hong 1,SUN Hai mei 1,GUO Chong jie 1* |
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| Abstract: | on splecytoes injury in mouse SIRS/MODS induced by lipopolysarchhride. Methods Morphology of spleen and apoptosis of splecytoes were compared in ginseng group with control by HE,TUNEL and transmission electromicroscope.Expression of Bcl 2,Fas,FasL were detected by immunocytochemical staining. Results Splecytoes injury increased with time in LPS group.Apoptotic cells increased in peri arteriolar lymphoid sheath as observed by transmission electromicroscope.The expression of Bcl 2 decreased with time,while the expression of Fas,FasL increased.Splecytoes injury decreased in ginseng group,as well as the increased expression of Bcl 2.Conclusion Ginseng abstract has protective effect on splecytoes injury induced by LPS,Bcl 2 may involve in this effect.;[ |
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| Keywords: | Ginseng Endotoxin Spleen Apoptosis Mice |
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