TNFA-308基因多态性与中国人群慢性牙周炎的相关性研究

目的：探讨TNFA～308基因多态性与慢性牙周炎易感性的关系。方法：收集63例重度慢性牙周炎（CP）患者、103例轻、中度慢性牙周炎患者及80例健康对照者的颊黏膜拭子，提取DNA，采用多聚酶链反应-限制性内切酶片段长度多态性（PCR—RFLP）法测定TNFA-308位点的基因多态性，实验结果输入SPSS10．0统计软件包处理，进行各组间基因型分布和等位基因频率的X^2检验，比较各组间基因型分布的差异。计算等位基因2的OR和95％可信区间，以确定等位基因2与牙周炎易感性的关系。结果：重度CP组、轻、中度CP组及健康对照组均以TNF1／1纯合子占优势，TNF1／2杂合子基因型次之，仅在轻、中度CP组检出1例TNF2／2纯合子基因型。TNFA-308／NcoI基因型分布及等位基因频率在3组间无显著性差异。结论：对此位点基因型是否对慢性牙周炎的易感性及进程预后产生影响。尚不能肯定，需要进一步的研究。

Association of TNFA-308 gene polymorphisms with susceptibility to chronic periodontitis in Chinese patients

PURPOSE: The purpose of this study was to investigate the relationship between TNFalpha-308 gene polymorphisms and susceptibility to chronic periodontitis in Chinese patients. METHODS: DNA samples with buccal swabs were collected from 63 severe CP patients, 103 initial to moderate CP patients, and 80 healthy controls in Chinese Han nationality. The TNFA-308 gene polymorphisms were analyzed with PCR-RFLP. The data were analyzed by X(2) test using SPSS10.0. RESULTS: There were no significant genotype distribution differences of TNFA-308 between either two of the three subject groups. CONCLUSION: Further study was needed since we did not find the relationship between TNFA-308 gene polymorphism and periodontitis in this present study.