急性冠状动脉综合征患者血清基质金属蛋白酶2水平的检测

目的检测急性冠状动脉综合征患者血清基质金属蛋白酶2的水平,探讨其与不稳定性斑块及临床危险度的内在联系。方法应用十二烷基硫酸钠—聚丙烯酰胺凝胶电泳及Western免疫印迹法分别检测急性冠状动脉综合症患者血清基质金属蛋白酶2的水平。其中不稳定型心绞痛组28例,分属低、中、高危3个亚组,急性心肌梗死组50例,正常对照组50例,稳定型心绞痛组51例。结果对照组、稳定型心绞痛组、不稳定型心绞痛组和急性心肌梗死组基质金属蛋白酶2水平分别为236±33 INT.mm2、224±23 INT.mm2、455±51 INT.mm2和503±45 INT.mm2。急性心肌梗死组和不稳定型心绞痛组分别与稳定型心绞痛组和对照组之间比较,基质金属蛋白酶2水平差异有显著性,对照组与稳定型心绞痛组之间基质金属蛋白酶2水平无统计学差异。4组之间血清基质金属蛋白酶原水平无明显差别。Western免疫印迹法也显示了类似的结果。不稳定型心绞痛低危、中危、高危3个亚组间比较,无论是酶原还是活性酶的水平均未发现显著差别。结论血清基质金属蛋白酶2水平在急性冠状动脉综合征患者中明显升高,提示血清基质金属蛋白酶2增高可作为急性冠状动脉综合症的一个危险信号,但基质金属蛋白酶2与急性冠状动脉综合症危险度并无相关性。

The Level of Matrix Metalloproteinase 2 in Patients of Acute Coronary Syndromes

Aim To investigate whether serum levels of matrix metalloproteinase(MMP) are associated with the degree of risk in patients with acute coronary syndromes(ACS). Methods Using SDS-PAGE Zymography and Western-Blotting,Serum Pro-MMP-2,MMP-2 were measured in 78 patients ACS,28 with unstable angina(UA) and 50 with acute myocardial infarction(AMI).Control group includes 50 healthy volunteers.51 cases were in stable angina group. Results Serum MMP-2 was significantly increased in both UA(455±51 INT·mm~2)and AMI group(503±45 INT·mm~2)compared with those in control group(236±33 INT·mm~2) and stable angina(224±23 INT·mm~2)(p<0.05,respectively)by Zymography.But there was no difference between UA and AMI group(p<0.05),and there were no difference between each two subgroups of UA(which are defined low-risk,mid-risk and high-risk group) in duration time of ischemia,ECG and CK-MB/cTnI. Conclusions Increased serum level of activity MMP-2 was detected in patients with ACS.Their findings provide an insight into the unstable plaque association with MMP-2.Serum MMP-2 level appears to be a marker of plaque unstable in patient with acute coronary syndrome.