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Effects of lung preservation solutions on PMN activation in vitro |
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| Authors: | F. Sakamaki Hans Hoffmann Silvia Münzing Fritz Krombach Konrad Messmer Friedrich W. Schildberg |
| Affiliation: | Department of medicine, National Cardiovascular Center, Fujishirodai 5-7-1, Suita-Shi, Osaka, 565-8565, Japan, JP Department of Surgery, Klinikum Gro?hadern, Ludwig Maximilians Universit?t München, Marchioninistrasse 15, D-81366 Munich, Germany, DE Institute for Surgical Research, Klinikum Gro?hadern, Ludwig Maximilians Universit?t München, Marchioninistrasse 15, D-81366 Munich, Germany, DE |
| Abstract: | Polymorphonuclear leukocyte (PMN) activation and PMN-endothelial cell interactions may cause graft failure due to ischemia-reperfusion injury after lung transplantation. We investigated the effects of Euro-Collins solution (EC), low-potassium dextran solution (LPD), and EC plus pentoxifylline (EC-PTXF) on adhesion molecule (CD11b/CD18 and L-selectin) expression, chemotaxis, and oxidative burst of PMN. PMN from healthy human volunteers were incubated with EC, LPD, and EC-PTXF, and, in controls, without preservation solution. LPD exerted a suppressive effect on PMN chemotaxis as compared to EC (P < 0.05), but had no attenuating effect on the increase of CD11b/CD18, the shedding of L-selectin, and intracellular oxidant generation. EC-PTXF attenuated the expression of CD11b/CD18 and the oxidative burst as compared to EC alone (P < 0.05). These effects of LPD and PTXF on PMN function may contribute to successful organ preservation in transplantation. Received 22 June 98 Received after revision: 30 December 1998 Accepted: 19 January 99 |
| Keywords: | Lung preservation solution neutrophil chemotaxis adhesion molecule |
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