Characterization of Borrelia burgdorferi sensu lato strains isolated from Ixodes ricinus in Mecklenburg-Vorpommern,Germany |
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| Authors: | Heike Lottmann Bettina Wilske H. Herrmann |
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| Affiliation: | Institut für Medizinische Mikrobiologie, Ernst-Moritz-Arndt-Universit?t Greifswald, Martin-Luther-Strasse?6, D-17487?Greifswald, Germany, Tel.: +49-3834/63516; Fax: +49-3834/63497, DE
Max von Pettenkofer Institut für Hygiene und Medizinische Mikrobiologie, Universit?t München, Pettenkoferstrasse?9?a, D-80336?Munich, Germany, DE
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| Abstract: | Epidemiological studies in Mecklenburg-Vorpommern have shown a high prevalence ofBorrelia burgdorferi-infected ticks. A total of 17B. burgdorferi sensu lato strains were isolated from ticks and investigated by Western blots (immunoblot) with eight monoclonal antibodies against different epitopes of the outer surface protein A (OspA). Except for one, all strains could be classified using this system. The majority of strains belonged to theB. garinii-associated OspA serotypes 3, 5 and 6. Three isolates were classified as OspA serotype 2 (B. afzelii).B. burgdorferi sensu stricto strains (Ospa serotype 1) as well asB. garinii-associated OspA serotype 4 were not present. |
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| Keywords: | Borrelia burgdorferi sensu lato Outer surface protein A Serotypes Monoclonal antibodies |
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