Genotoxicity of crude extracts of cyanobacteria from Taihu Lake on carp (<Emphasis Type="Italic">Cyprinus carpio</Emphasis>) |
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| Authors: | Qin Wu Mei Li Xiangyu Gao John P Giesy Liuyan Yang Zhiming Kong |
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| Institution: | (1) State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Xianlin Campus, 163 Xianlin Avenue, Nanjing, 210046, People’s Republic of China;(2) Department of Biomedical Veterinary Biosciences and Toxicology Centre, University of Saskatchewan, Saskatoon, SK, S7N 5B3, Canada;(3) Zoology Department, Center for Integrative Toxicology, Michigan State University, East Lansing, MI 48824, USA;(4) Biology and Chemistry Department, City University of Hong Kong, Kowloon, Hong Kong SAR, China; |
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| Abstract: | Genotoxicity of crude cyanobacteria extracts (CBE) from blooms in Taihu Lake, China toward common carp (Cyprinus carpio) was measured. The primary extracellular product was determined by HPLC to be Microcystin-LR (MC-LR, L for leucine and R
for arginine) with an average concentration of 2.4 × 102 μg MC g−1 dry weight of cyanobacteria. Acute toxicity to carp, expressed as the 72-h LC50, was 53 mg, dw cyanobacteria L−1. Genotoxicity, as determined by the micronucleus (MN) and comet assays were both dose- and time-depended. Deformities of
cellular organelles in liver and gill were observed by use of transmission electron microscopy (TEM). The results showed that
MC-LR from cyanobacteria from Taihu Lake could induce genotoxic response and tissue-level morphological changes in common
carp. |
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