抑制ClC-3表达促进顺铂诱导人卵巢癌SKOV3/DDP细胞凋亡的作用机制

目的:探讨抑制ClC-3基因表达对顺铂(DDP)复制的人卵巢癌SKOV3/DDP细胞损伤促进作用过程中的具体作用机制,为应用DDP治疗卵巢癌提供实验依据。方法:转染pSH1-siRNA-ClC-3重组质粒到人卵巢癌SKOV3/DDP细胞,Western blotting方法观察热休克蛋白(HSP70)、溶酶体组织蛋白酶D(cathepsin D)蛋白表达。结果:与SKOV3细胞比较,SK-OV3/DDP细胞中HSP70蛋白表达增加(P<0.05)。转染pSH1-siRNA-ClC-3重组质粒联合DDP作用后,SKOV3/DDP细胞中HSP70蛋白表达降低(P<0.05),cathepsin D蛋白表达增加(P<0.05)。结论:抑制ClC-3基因表达促进顺铂诱导SKOV3/DDP细胞凋亡的可能作用机制与溶酶体膜通透性(LMP)增加有关。

Mechanism of promoting effect of inhibition of ClC-3 expression on apoptosis of human ovarian cancer SKOV3/DDP cells induced by cisplatin

Objective:To explore the specific mechanism of promoting effect of inhibition of chloride channel-3(ClC-3) expression on human ovarian cancer SKOV3/DDP cell injury induced by cisplatin,and provide an experimental basis for treating ovarian cancer with DDP.Methods:pSH1-siRNA-ClC-3 recombinant plasmid was transfected into human ovarian cancer SKOV3/DDP cells,Western blotting was used to observe the expressions of heat shock protein 70(HSP70) and lysosomal cathepsin D(cathepsin D).Results:Compared with SKOV3 cells,HSP70 expression increased in SKOV3/DDP cells(P<0.05).HSP70 expression decreased(P<0.05) and cathepsin D expression increased in human ovarian cancer SKOV3/DDP cells after treated with transfection of pSH1-siRNA-ClC-3 recombinant plasmid combined with DDP.Conclusion:The mechanism of promting effect of inhibition of ClC-3 expression on apoptosis of human ovarian cancer SKOV3/DDP cells induced by cisplatin may be related to increase of permeability of lysosomal membrane.