胃癌脉管转移与细胞增殖活性关系的探讨

目的探讨胃癌脉管转移与细胞增殖活性的关系。方法用溴化脱氧脲嘧啶核苷(BrdUrd)/DNA双参数流式细胞术对60例胃癌新鲜标本进行检测,研究胃癌细胞BrdUrd标记指数(BrdUrdLI)、胃癌G2/M期细胞比率(G2/M-fraction,G2/MPF)和DNA含量与胃癌静脉浸润、淋巴管浸润及淋巴结转移的关系。结果静脉癌栓阳性较阴性者G2/MPF明显增高(P<0.01),淋巴管癌栓阳性较阴性者BrdUrdLI、G2/MPF均明显增高(P<0.01)。淋巴结阳性较阴性者BrdUrdLI、G2/MPF均明显增高(P<0.01)。异倍体癌淋巴结阳性者明显增多(P<0.05)。淋巴结转移第Ⅱ站以远(N2↑)者,BrdUrdLI、G2/MPF均较无转移者明显增高(P<0.01);淋巴结转移第Ⅰ站者,G2/MPF较无转移者明显增高(P<0.01)。异倍体癌淋巴结转移N2↑者明显增多(P<0.01)。结论胃癌细胞BrdUrdLI、G2/MPF和DNA含量与脉管浸润有关。

Study on the relationship between cellular proliferative activity and lymphatic and vascular invasions in gastric carcinoma

Objective To evaluate BrdUrd/DNA double parametric method and to study the relations of cellular BrdUrd labeling index(BrdUrd LI),G2/M fraction(G2/MPF) and DNA content with lymphatic and vascular invasions in gastric carcinoma. Methods Fresh tumor samples from sixty cases were examined by BrdUrd/DNA doubleparametric flow cytometry. Results G2/MPF was significantly higher in the cases with vascular invasion than those without invasion(P< 0.01). BrdUrd LI and G2/MPF values were both significantly higher in the cases with lymphatic invasion than those without invasion (P< 0.01), and also in the cases with lymphatic node metastasis than those without metastasis (P< 0.01). The incidence of lymphatic node metastasis was significantly higher in aneuploid carcinomas than that in diploid carcinomas (P< 0.05). BrdUrd LI and G2/MPF values were both significantly higher in the cases with N2 lymph metastasis than those with no metastasis(P< 0.05). G2/MPF was significantly higher in the cases with N1 lymphatic metastasis than those with no metastasis(P< 0.01). Conclusion Cellular BrdUrd LI, G2/MPF, DNA content may be correlated with lymphatic, vascular invasions in gastric carcinoma.