肺癌支气管冲洗液微卫星DNA的检测及意义

目的　探索肺癌支气管冲洗液中微卫星DNA的改变及其在肺癌诊断中的价值。方法　收集肺癌及良性肺病患者支气管冲洗液和外周血 ,提取DNA ,利用聚合酶链反应、变性聚丙烯酰胺凝胺电脉及银染法检测冲洗液中的微卫星DNA改变 ,并与纤维支气管镜活检 ,刷检作比较。结果　肺癌冲洗液中微卫星DNA改变阳性率5 8% ,其中中心型 5 6% ,低于活检及刷检 ;周围型 61% ,高于活检及刷检。良性肺病未发现微卫星DNA改变。结论　肺癌支气管冲洗液微卫星DNA改变的检测阳性率较高 ,特异性好 ,对于肺癌尤其周围型肺癌的早期诊断具有较好的应用价值

The detection of microsatellite DNA in bronchial washing fluids of lung cancer and its clinical significance

Objective To explore the alteration of microsatellite DNA in bronchial washing fluids (BWF) of lung cancer and its diagnostic value.Method The BWF and blood of patients with lung cancer and benign pulmonary disorder were collected.The alterations of microsatellite DNA in BWF were detected by PCR?denaturing polyacrylamide gels and siver staining and were compared with fiberoptic bronchoscopic biopsy and brushing.Result The positive rate of microsatellite DNA alteration in BWF of lung cancer was 58%.In central lung cancer it was 56%,lower than biopsy and brushing;but in peripheral lung cancer it was 61%,higher than biopsy and brushing.No alteration of microsatllite DNA was found in benign pulmonary disorder.Conclusion The microsatellite DNA alteration in BWF of lung cancer has high sensitivity and specificity,so it is valuable in early diagnosis of lung cancer,especially peripheral lung cancer.