| 血红素加氧酶-1介导Tr1细胞在哮喘动物模型中的免疫调节作用 |
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| 引用本文： | 蔚京京,钟文伟,须丽清,邵洁,李云珠,俞善昌,周光炎,夏振炜. 血红素加氧酶-1介导Tr1细胞在哮喘动物模型中的免疫调节作用[J]. 临床儿科杂志, 2009, 27(1) |
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| 作者姓名： | 蔚京京 钟文伟 须丽清 邵洁 李云珠 俞善昌 周光炎 夏振炜 |
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| 作者单位： | 上海交通大学医学院附属瑞金医院儿科,上海,200025;上海市免疫学研究所,上海,200025 |
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| 基金项目： | 国家自然科学基金，上海市科学技术委员会资助项目，上海市教委资助项目 |
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| 摘 要： | 目的 探讨血红素加氧酶-1(heme oxygenase-1,HO-1)介导1型T调节细胞(type 1 T regulatory cells,Tr1)在哮喘动物模型中的免疫调节作用.方法 用卵清蛋白(ovalbumin,OVA)致敏、激发C5786小鼠建立哮喘模型,并在致敏、激发阶段分别经血红素(hemin)和锡-原卟啉(Sn-protoporphyrin,SnPP)干预.肺泡灌洗液细胞计数和肺脏病理组织学检测评价哮喘动物模型;real-time PCR和Western blot方法分别测定脾脏细胞内HO-1、IL-10mRNA表达和HO-1蛋白量;ELISA方法测定动物血清OVA-特异性IgE(OVA-sIgE)和IL-10水平;流式细胞计数测定脾脏细胞中CD4+CD25+IL-10+Treg、CD4+IL-10R+IFN-γR+Tr1的比例.结果 哮喘小鼠模型经hemin干预后,Tr1数量增加,脾脏细胞内HO-1和IL-10 mRNA表达上调,HO-1蛋白水平明显增加,血清IL-10水平增高,哮喘气道炎症减轻.结论 在哮喘动物模型中HO-1可能通过诱导Tr1细胞,增加IL-10的分泌,抑制哮喘气道炎症.
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| 关 键 词： | 血红素加氧酶-1 哮喘小鼠模型 调节性T细胞 IL-10 |
Heme oxygenase-1 mediates immunoregulation of Tr1 cells in asthmatic animal model |
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| WEI Jing-jing,ZHONG Wen-wei,XU Li-qing,SHA Jie,LI Yun-zhu,YU Shan-chang,ZHOU Guang-yan,XIA Zhen-wei. Heme oxygenase-1 mediates immunoregulation of Tr1 cells in asthmatic animal model[J]. The Journal of Clinical Pediatrics, 2009, 27(1) |
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| Authors: | WEI Jing-jing ZHONG Wen-wei XU Li-qing SHA Jie LI Yun-zhu YU Shan-chang ZHOU Guang-yan XIA Zhen-wei |
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| Affiliation: | 1. Department of Pediatrics;Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine;Shanghai 200025;China;2. Shanghai Institute of Immunology;China |
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| Abstract: | Objectives To explore the immunosuppressive function of type 1 T regulatory (Tr1) cells after treated with heme oxygenase-1 (HO-1) in asthmatic mouse model. Methods C57B6 mouse was sensitized and challenged by ovalbumin (OVA) and administrated with hemin or Sn-protoporphyrin (SnPP). Airway inflammation was analyzed by cell counting of bronchoalveolar lavage fluid (BALF) and lung histopathology. The expression levels of HO-1, IL-10 mRNA and protein were detected in splenic cells. The levels of OVA-specific I... |
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| Keywords: | IL-10 |
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