直肠癌的血管生成和血管内皮生长因子表达的实验研究

目的　探讨直肠癌组织微血管密度 (MVD)和血管内皮生成因子 (VEGF)表达与肿瘤浸润和转移的关系 .方法　应用CD31抗体和VEGF抗体 ,采用免疫组化S -P法对 14 1例手术切除的直肠癌患者进行血管标记和染色 ,并取 10例直肠正常组织对照 .结果　有淋巴转移组直肠癌MVD、VEGF表达强度与无淋巴结转移组、正常对照组间比较 ,均有显著性差异 (p<0 .0 1) ,且MVD与VEGF表达两者呈正相关 (r=0 .93) .结论　直肠癌组织内微血管密度和VEGF表达强度与直肠癌的浸润深度及淋巴结转移密切相关 ,两者均可考虑作为预测直肠癌发生转移的指标 .

A Study on Tumor Angiogenesis and Expression of VEGF in Rectal Cancer

Objective To evaluate the relationship between tumor microvessel density and VEGF status and rectal neoplasms metastasis. Methods The microvessel density(MVD) and the level of vascular endothelial groth factor (VEGF) expression were examined imunohistochemically from 141 specimens obtained by surgical resection .Results MVD and the expression of VEGF were both significantly higher in the group of lymph node metastasis than that in the group of lymph node negative and normal control. MVD in tumor is associated with VEGF expression in rectal neoplasms. Conclusion Microvessel density and VEGF expression in rectal neoplasms are highly correlated with the depth of tumor invasion and lymph node metastasis,which may serve as a parameter for determining rectal neoplasms metastasis.