不同钛表面对血管内皮细胞早期粘附与增殖活性的影响

目的:观察血管内皮细胞在两种纯钛表面(光滑表面和粗糙表面)上的粘附情况.方法:医用纯钛片预备成光滑表面和粗糙表面两组,将猪血管内皮细胞接种于两组钛片表面,分别培养24、48和72 h后,通过吖啶橙染色和MTT活性测定,观察细胞在两种不同表面上的粘附和增殖活性.结果:荧光显微镜显示两组钛片上的内皮细胞均随着培养时间延长而增多,24 h时粗糙表面钛片上粘附的内皮细胞明显多于光滑表面组,而且细胞形态伸展好、成梭形.MTT结果显示粗糙表面组24 h和48 h的0D490值显著高于光滑表面组,而在培养72 h时,两组间没有统计学差异.结论:纯钛经双酸蚀处理形成的粗糙表面较其光滑表面更有利于血管内皮细胞的早期粘附,并促进其增殖.

Effect of Surface Characteristics of Pure Titanium on the Adhesion of Vascular Endothelial Cells

Objective: To observe the adhesion of vascular endothelial cells on two types of pure titanium surface(smooth surface and rough surface).Methods: Pure titanium plates were prepared with smooth surface and rough surface.Endothelial cells were cultured on the surface of two groups for 24,48 and 72 hours.The fluorescent stain and MTT methods were used to observe the adhesion and activity of adhesion cells.Results: Fluorescence microscopy showed the adhesion cells increased as the culture time elapsed.The adhered endothelial cells on the rough surfaces were obviously more than those on the smooth surfaces at 24 hours,and the adhesion cells spread into fusiform shape.MTT results revealed that the OD490 value on rough surface was significantly higher than that on smooth surface at 24h and 48h,while there was no difference between two groups at 72h.Conclusion: Rough implant surface treated by double acid-etching is more beneficial for early adhesion of vascular endothelial cells.