癫痫大鼠海马超氧化物歧化酶与一氧化氮关系的研究

目的：观察癫痫大鼠海马组织超氧化物歧化酶（SOD）与海马一氧化氮（NO）的变化，并探讨二者之间的关系。方法：通过贝美格制成癫痫发作动物模型，分别取发作5、30min及注药6、12、24h的海马组织，硝酸还原酶法、黄嘌呤、氧化酶法分别测NO、SOD。结果：海马组织SOD于发作5min时显著低于正常对照组；6h时仍与对照组存在差异；12h后差异不显著；海马组织NO在发作5min迅速升高，30min组仍高于正常对照组，6h后与正常对照组比无显著差异。经相关统计学检验，SOD与NO之间呈显著负相关（γ=-0.849，P＜0．01）。结论：癫痫发作可导致海马自由基清除剂SOD严重损伤，SOD活力降低与NO之间存在相关关系。

The relationship between hippocampal superoxide dismustase and nitric oxide inepileptic rats induced by bemegride

Objectives:To study the changes of superoxide dismustase(SOD) and nitric oxide (NO) in hippocampus of epileptic rats and to analyze the relationship between SOD and NO. Methods:The animal mode of epileptic rat was made by intraperitoneal injection of bemegride in a dose of 25mg/kg.The concentrations of SOD and NO in hippocampus of epileptic rats were measured by nitroreductase and xanthylicoxidase at 5 and 30 minutes after seizure and at 6, 12 and 24 hours after the injection of bemegride.Results:SOD was found to be the lowest at 5 minutes after the seizure and was still lower than the controls at 6 hours after the injection of bemegride. It returned to normal at 12 hours after the injection of bemegride. NO levels were increased greatly at 5 minutes after the seizure and was still higher than that in the controls 30 minutes after the seizure. It returned to normal at 6 hours after the injection of bemegride. There was a negative correlation( r= -0.849 , P <0.01) between SOD and NO in hippocampus. Conclusions:The decrease of SOD induced by seizure was found to be related to the increase of NO in hippocampus.