乌司他丁联合甲基泼尼松龙对大鼠肺缺血再灌注损伤的保护作用

目的探讨乌司他丁联合甲基泼尼松龙对大鼠肺缺血再灌注损伤时血清水通道蛋白-1(AQP 1)及肿瘤坏死因子α(TNF-α)的影响。方法将40只Wistar大鼠随机分为5组(n=8):正常对照组、模型对照组、甲基泼尼松龙组(30 mg/kg)、乌司他丁组(50 000 U/kg)及联合治疗组甲基泼尼松龙(30 mg/kg)联合乌司他丁(50 000 U/kg)]。采用ELISA法检测血清AQP 1和TNF-α水平;考马斯亮蓝法检测肺泡灌洗液中蛋白质含量;检测肺湿/干质量并对肺进行形态学观察。结果与模型对照组相比,乌司他丁组或甲基泼尼松龙组血清AQP 1质量浓度明显增加,而TNF-α质量浓度明显降低,联合治疗组血清AQP 1与TNF-α的改变更为明显(P<0.01);乌司他丁组、甲基泼尼松龙组及联合治疗组的肺泡灌洗液中蛋白质含量及肺组织湿/干质量比值均明显降低(P<0.01)。结论乌司他丁联合甲基泼尼松龙对大鼠缺血再灌注急性肺损伤具有良好的治疗作用,其机制可能与提高血清AQP 1水平及降低TNF-α水平有关。

Protective effects of ulinastatin combined with methylprednisolone on pulmonary ischemia reperfusion injury in rats

Objective To explore the influence of ulinastatin combined with methylprednisolone on aquaporin 1(AQP 1) and tumor necrosis factor-alpha(TNF-α) in serum when ischemia reperfusion injury occured in rats.Methods 40 Wistar rats were randomly devided into 5 groups(n=8):normal control group,model control group,methylprednisolone group(30 mg/kg),ulinastatin group(50 000 U/kg) and combination groupulinastatin(50 000 U/kg) combined with methylperdnisolone(30 mg/kg)].Levels of AQP 1 and TNF-α in serum were detected by enzyme-linked immunosorbent assay(ELISA);Protein contents in bronchoalveolar lavage fluid were assayed by Commassie blue staining;Wet and dry mass were measured by scale and pulmonary morphological changes were observed by optical microscope.Results Compared with model control group,serum concentration of AQP 1 was significantly increased and that of TNF-α decreased in ulinastatin group and methylprednisolone group,especially in combination group(P<0.01);Protein contents in bronchoalveolar lavage fluid and wet/dry mass ratios were both markedly reduced in ulinastatin,methylprednisolone and combination groups(P<0.01).Conclusion Ulinastatin combined with methylprednisolone administration has better therapeutic effects on acute lung injury caused by ischemia reperfusion in rats,which mechanism probably correlated with increasing level of serum AQP 1 and decreasing that of TNF-α.