龙血竭小鼠灌胃给药后血中移行成分的分析

目的 对龙血竭进行中药血清药物化学研究,为确定龙血竭的药效成分奠定基础.方法 在建立龙血竭高效液相色谱指纹图谱的基础上,通过比较龙血竭提取物、含药血清和空白血清的HPLC指纹图谱,结合LC-MS/MS检测技术确定龙血竭给药后的血中移行成分.结果 在龙血竭含药血清中发现6个人血成分,其中5个都属原型成分,分别为3,4'-二羟基-5-甲氧基二苯乙烯、剑叶龙血素B、4'-羟基-4,2'-二甲氧基一二氢查耳酮(新化合物)、剑叶龙血素A和龙血素B;1个可能为原型成分或代谢产物.结论 6个人血成分是龙血竭在体内主要直接作用物质,有助于阐明龙血竭的药效物质基础.

Analysis on migrating constituents absorbed into blood of mice after ig administration of Resina Draconis

Objective Serum pharmacochemistry was performed to screen the bioactive constituents of Resina Draconis.Methods Based on HPLC fingerprints of Resina Draconis,the migrating constituents absorbed into blood were determined by comparing the HPLC fingerprints of extraction of Resina Draconis(ERB),herb serum sample,and control serum sample,and with the help of LC-MS/MS.Results Six compounds absorbed into blood were detected,five of them were original in form which were 3,4'-dihydroxy-5-methoxystilbene,cochinchinenin B,4'-hydroxy-4,2'-dimethoxy-dihydrochalcone(a new compound),cochinchinenin A,and loureirin B,respectively.The other might be the original constituent or the metabolite.Conclusion The six constituents absorbed into blood are possible bioactive components of Resina Draconis in vivo.Further research will help clarify the bioactive constituents and mechanisms of Resina Draconis.