| 高效液相色谱法测定儿童Ⅱ号中龙胆苦甙的含量 |
| |
| 引用本文： | 梁小庆 石涛 王世宏 刘炯 申兰慧. 高效液相色谱法测定儿童Ⅱ号中龙胆苦甙的含量[J]. 实用中西医结合临床, 2004, 4(5): 71-72 |
| |
| 作者姓名： | 梁小庆 石涛 王世宏 刘炯 申兰慧 |
| |
| 作者单位： | 1. 东南大学附属中大医院药剂科,江苏,南京,210009
2. 中国药科大学药物分析教研室,江苏,南京,210009 |
| |
| 摘 要： | 目的建立测定儿童Ⅱ号中的龙胆苦甙含量的方法。方法采用高效液相色谱法,以DiamonsilTM–C18(4.6×200mm,5μm)为色谱柱,以乙腈水(1288)流动相流速1.0mL/min,检测波长270nm,外标法定量。结果此法线性关系良好,平均加样回收率为98.94%RSD为2.95%重复性RSD为1.49%。结论本方法精密度高,分离度良好,可为测定儿童Ⅱ号中龙胆苦甙的含量提供可靠的分析方法。
|
| 关 键 词： | 龙胆苦甙 高效液相色谱法 含量测定 儿童II号 性早熟 中药疗法 |
| 文章编号： | 1671-4040(2004)05-0071-02 |
| 修稿时间： | 2004-07-10 |
Content Determination of Gentiopicrin in Er-tong Ⅱ by HPLC |
| |
| Liang Xiao-qiu,Shi Tao,Wang Shi-hong,etal. Content Determination of Gentiopicrin in Er-tong Ⅱ by HPLC[J]. Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine, 2004, 4(5): 71-72 |
| |
| Authors: | Liang Xiao-qiu Shi Tao Wang Shi-hong etal |
| |
| Abstract: | Objective:To establish a method for determination of content of gentiopicroside in er tongⅡ.Methods:HPLC was adopted,Diamonsil TM-C18(200mm×4.6mm) was used,the mobile phase was acetonitrile-water(12:88),The wavelength was at 270nm. The quantitative determination of gentiopicroside was performed with external standard method. Results:the method has a good linear ; The average recovery rate for gentiopicroside was 98.94% and RSD was 2.95%. The RSD of the reproducibility was1.49%.Conclusions: The precision was high; The separation method is accurate, reproducible and highly selective. The method used for the determination of gentiopicroside in er tongⅡ is quite better. |
| |
| Keywords: | Gentiopicroside HPLC Detemination |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《实用中西医结合临床》浏览原始摘要信息 |
|
点击此处可从《实用中西医结合临床》下载免费的PDF全文 |
|
