东海刺海参冻干粉对荷瘤小鼠的抑瘤作用及其免疫指标的影响

目的研究东海刺海参冻干粉的抑瘤作用,并从免疫学角度初步探讨其抑瘤机制。方法健康昆明小鼠50只,皮下接种S180肿瘤细胞,次日随机分成模型对照组、阳性对照组(20mg/kg)和低、中、高3个剂量组(0.4g/kgbw,0.8g/kgbw,1.6g/kgbw),连续给予受试物10天后,观察(1)瘤重、抑瘤率;(2)瘤组织的病理改变,免疫组化法测定瘤组织中caspase-3蛋白的表达;(3)脾指数、胸腺指数;(4)利用酶联免疫吸附试验法检测血清中白介素-2(IL-2)的含量。结果(1)海参各剂量组与模型对照组比较瘤重明显减小,抑瘤率显著增加(P<0.05或P<0.01)。(2)模型对照组的病理改变较之中高剂量组非常明显,各剂量组caspase-3蛋白表达明显高于模型对照组(P<0.01),低于阳性对照组。(3)脾指数、胸腺指数有一定的增加趋势,但无统计学意义。(4)IL-2的含量在各剂量组中逐渐增加,与模型对照组相比则显著增加(P<0.01)。结论刺海参冻干粉有明显的抗肿瘤作用,对免疫系统具有一定的保护和恢复作用。

Effect of freeze-dried sea cucumber powder of eastern sea on tumor and immune index of S180-bearing mouse

OBJECTIVE: To study the effect of freeze-dried sea cucumber powder from eastern sea on tumor and immune of S180-bearing mouse. METHODS: S180 tumor cells were subcutaneouly inoculated to fifty health kunming mice and were randomly divided into five groups (model control group, positive control group, and low, medium, high dose groups). After the groups were orally treated with solution of freeze-dried sea cucumber powder for ten days,the weights of tumors and the inhibition rates of tumor were counted, and the pathological changes of tumors were observed and the expressions of caspase-3 proteins were detected in the tumor tissues, and the thymus index and spleen index were observed , the contents of interleukin-2(IL-2) in the serum were detected. RESULTS: (1) In comparison with the control groups,the weights of tumor were declined and the inhibition rates of tumor were remarkably decreased in the treated groups. (2) The pathological changes of model control group were very obvious. The expressions of caspase-3 proteins in the treated group were more higher than those of model control groups, while were more lower than those of positive control groups. (3) The thymus index, spleen index in the treated group indicated an increased tendency. (4) The contents of IL-2 in the treated groups were more higher than those of other groups. CONCLUSION: Freeze-dried sea cucumber powder could be effective antitumor and could protect and recovery the immune system of S180-bearing mice.