Studies on morphology and aristolochic acid analogue constituents of <Emphasis Type="Italic">Asarum campaniflorum</Emphasis> and a comparison with two official species of Asari Radix et Rhizoma |
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Authors: | Yao-Li Li Min Tian Jie Yu Ming-Ying Shang Shao-Qing Cai |
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Institution: | (1) State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xue-yuan Road, Haidian District, Beijing, 100191, China; |
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Abstract: | Dried whole herbs or roots and rhizomes of Asarum campaniflorum have been sold under the trade name Xixin and used as folk remedies in its producing areas for a long time. In order to avoid
the misuse of A. campaniflorum as official species of Xixin (Asari Radix et Rhizoma), a comparative study based on the morphological and phytochemical analysis
of the aerial and underground parts was carried out. The usual morphological methods and a microscopic imaging system were
used. The results show that A. campaniflorum could be easily distinguished from two official species (Asarum sieboldii and A. heterotropoides var. mandshuricum) by the diameter of thicker roots (1.3–2.7 mm), distinct large parenchymatous cells in phloem of roots, and the size of oil
cells in upper leaf epidermises (40)80–140(174) μm in diameter, where numbers in parentheses are for rare cases], etc. Nine
aristolochic acid analogues (AAAs) were identified and estimated by high-performance liquid chromatography–diode array detection
(HPLC-DAD). The aerial and underground parts of A. campaniflorum contained 3–4 AAAs, i.e., aristololactam-II-N-β-d-glucoside (AL-II-Glc), aristololactam I (AL-I), aristololactam II (AL-II), and aristolochic acid I (AA-I), while only AL-I
was detected in underground parts of A. sieboldii and no AAAs were detected in underground parts of A. heterotropoides var. mandshuricum. The respective contents (in mg/g) in aerial and underground parts of A. campaniflorum, were as follows: AL-I, 0.06–0.12, 0.05–0.10; AL-II, 0.03–0.04, 0.01–0.03; AA-I, 0.01–0.02, 0.0–0.0. These data suggest A. campaniflorum has a high risk of causing aristolochic acid nephropathy. All these discoveries can contribute to not only the better understanding
of this new resource species, but also the safe use of the crude drug Xixin. |
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