脑缺血后不同再灌注条件对大鼠脑ASICs3表达的影响

目的]研究SD大鼠脑在缺血后不同再灌注流量时可渗性钙离子酸敏感离子通道(ASICs)的表达特点.方法]SD大鼠随机分3组,A组(适应性再灌注组,n=15)为缺血后逐步开放脑血流量10 min,B组(一次性再灌注组,n=15)为缺血后完全开放脑血流量10 min,C组(对照组)为缺血重度损伤组.分别观察海马CA区ASICs的表达,脑血流量的变化及缺血大脑半球的pH改善状况和海马区形态学改变.结果]重度脑损害后A组再灌注流量由(0.5±0.1)mL/min逐渐增加到(3.2±0.6)mL/min,B组再灌注流量由(0.5±0.1)mL/min增加到(6.2±1.5)mL/min,灌注流量明显增加.A组pH值改善最为明显,由7.15±0.05上升到7.38±0.04(P<0.05),而B组pH值由7.15±0.05变为7.10±0.04,A组pH值与B、C组相比差异有显著性意义,P<0.05.B组颈总动脉脑血流量值与A、C组相比差异有显著性意义,P<0.05.海马CA区ASICs的表达B组较A组明显上调,而A组可以看到较B组更多密集的正常神经元细胞.结论]ASICs3的表达和脑缺血不同再灌注方式有相关性,适应性再灌注流量有较轻的脑损害作用.

Influencing of ASICs3 expression under different reperfusion condition after SD rats brain ischemia

Objective] The purpose of the study was to evaluate the role of ASICs in the different reperfusion inflow in SD rats,and the relationship between acidosis and ASICs after global ischemic insults.Methods] The SD rats ischemic models were divided into three groups randomly:group A:adaptive ischemia-reperfusion group;group B:one-time ischemia-reperfusion group;group C: severe cerebral ischemic injury group.The SD rats were made by bilateral carotid artery 10 min occlusion.After the whole reperfusion 10 min a...