生骨再造散对兔骨折愈合影响的实验研究

目的探讨中成药生骨再造散对兔实验性骨折愈合的影响. 方法将30只青紫蓝家兔制成双侧桡骨骨膜下3 mm骨缺损模型,随机分为生骨再造散组(A组)、仙灵骨葆组(B组)及对照组(C组),每组10只,于术后14天和31天每组各处死5只兔,按规定时间进行四环素双标记(即14天处死的兔于术后4、5天行第1次标记,术后11、12天行第2次标记;31天处死的兔于术后20、21天行第1次标记,术后28、29天行第2次标记),并分别取材,进行骨组织形态学计量检测. 结果术后14天A组与C组比较,类骨质面积密度、矿化骨痂均宽及活性成骨面、矿化表面及动态参数等差异均有统计学意义(P＜0.01);术后31天,活性成骨面、矿化骨痂均宽及吸收陷窝均深等差异均有统计学意义(P＜0.01).B组与C组比较,结果与A组和C组比较类似;但与A组比较,术后14天破骨细胞指数、矿化表面及骨形成率[组织水平(tissue level)及BMU水平(basic multicellular unit level)]差异均有统计学意义(P＜0.05),术后31天类骨质表面、吸收陷窝均深、骨形成率(BMU水平)差异均有统计学意义(P＜0.05). 结论生骨再造散在兔桡骨骨折14天前可促进类骨质分泌,加快矿化沉积速度,提高骨形成率,缩短矿化延迟时间;14～31天后则可增加矿化骨痂宽度,提高破骨细胞活性,对骨折愈合有促进作用.

EXPERIMENTAL STUDY ON EFFECT OF SHENGGUZAIZAOSAN ON RABBITS FRACTURE HEALING

Objective To study the effect of Shengguzaizaosan on experimental fracture healing. Methods Thirty six month old Chinchilla rabbits were made the models with 3 mm bone defect in the middle of two side radius and were divided randomly into 3 groups: Shengguzaizaosan group(group A, n =10), Xianlinggubao group(group B, n =10) and control group(group C, n =10). Five rabbits were sacrificed 14 days and 31 days after operation respectively. The double marks with tetracyslin hydrochloride were given on the 4th day and the 5th day for the first mark and on the 11th day and the 12th day for the second mark in the rabbits sacrificed 14 days later, and on the 20th day and the 21st day for the first mark and on the 28th day and the 29th day for the second mark in the rabbits sacrificed 31 days later. The samples were taken to make the bone histomorphometry study. Results When compared group A with group C, there were significant differences( P<0 01) in osteoid area density, osteoid mean width, active osteoblastic surface, mineralization surface and dynamic parameters after 14 days,and in mean width of mineralization callus, active osteoblastic surface and mean depth of lacunae after 31 days. There was a approximate effect between group B and group C. when compared group B with group A, there were significant differences( P<0.05) in osteoclastic index,mineralization surface,bone formation rate after 14 days, and in osteoid surface,mean depth of lacunae and bone formation rate after 31 days. Conclusion Shengguzaizaosan can increase osteoid, accelerate mineralization rate and bone formation rate, and reduce mineralization lag time at early period of fracture. It can increase mineralization callus width and resorption of osteoclast at later period of fracture. To some extent, shengguzaizaosan can accelerate the experimental fracture healing.