Interleukin 28B-related polymorphisms: A pathway for understanding hepatitis C virus infection? |
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引用本文: | Raquel Francine Liermann Garcia,Simone Moreira,Ana Lucia de Araújo Ramos,Leslie Ecker Ferreira,Angelo Alves de Mattos,Cristiane Valle Tovo,Lysandro Alsina Nader,Juliene Antonio Ramos,Edson Rondinelli,Arnaldo de Jesus Dominici,Christian Evangelista Garcia,Mauro de Souza Leite Pinho,Carlos Eduardo Brando-Mello,Cristiane Alves Villela-Nogueira,Paulo Henrique Condeixa de Frana.Interleukin 28B-related polymorphisms: A pathway for understanding hepatitis C virus infection?[J].世界胃肠病学杂志(英文版),2013,19(42):7399-7404. |
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作者姓名: | Raquel Francine Liermann Garcia Simone Moreira Ana Lucia de Araújo Ramos Leslie Ecker Ferreira Angelo Alves de Mattos Cristiane Valle Tovo Lysandro Alsina Nader Juliene Antonio Ramos Edson Rondinelli Arnaldo de Jesus Dominici Christian Evangelista Garcia Mauro de Souza Leite Pinho Carlos Eduardo Brando-Mello Cristiane Alves Villela-Nogueira Paulo Henrique Condeixa de Frana |
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作者单位: | Departamento de Medicina,Universidade da Regio de Joinville/Univille,Joinville,89219-719 Santa Catarina,Brazil;Departamento de Clinica Médica e Cirurgia Geral Hospital Municipal So José,Joinville,89202-000 Santa Catarina,Brazil;Departamento de Clínica Médica,Servio de Hepatologia,Faculdade de Medicina/Hospital Universitário Clementino Fraga Filho/Universidade Federal do Rio de Janeiro,21941-913 Rio de Janeiro,Brazil;Servio de Gastroenterologia e Hepatologia,Irmandade da Santa Casa de Misericórdia/ISCMPA,Porto Alegre,90020-090 Rio Grande do Sul,Brazil;Servio de Endoscopia,Universidade Federal de Pelotas/UFPel,Pelotas,96010-610 Rio Grande do Sul,Brazil;Instituto de Biofísica Carlos Chagas Filho/UFRJ,21941-902 Rio de Janeiro,Brazil;Departamento de Gastroenterologia,Hospital Universitário da Universidade Federal do Maranh o/UFMA,So Luis,65020-560 Maranho,Brazil;Servio de Gastroenterologia,Hospital Universitário Gaffrée Guinle/HUGG/UNIRIO,20270-004 Rio de Janeiro,Brazil |
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基金项目: | Supported by Grants from the Research Fund from University of Region of Joinville,FAP-UNIVILLE |
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摘 要: | AIM:To analyze the role of rs12979860 and rs8099917polymorphisms in hepatitis C virus(HCV)genotype 1infection of Brazilians.METHODS:A total of 145 adult patients diagnosed with genotype 1 chronic hepatitis C(CHC)who had completed a 48-wk regimen of pegylated-interferonα-2a or-2b plus ribavirin combination therapy were recruited from six large urban healthcare centers and199 healthy blood donors(controls)from a single site between January 2010 and January 2012.Data on the patients’response to treatment was collected.Polymerase chain reaction-restriction fragment length polymorphism genotyping of the interleukin(IL)28B gene fragment encompassing the single nucleotide polymorphisms(SNPs)rs12979860(C/T)and rs8099917(T/G)was carried out for 79 of the CHC patients and 199 of the controls.Bi-directional amplicon sequencing of the two SNPs was carried out for the remaining 66 CHC patients.RESULTS:SNP rs12979860 genotyping was successful in 99.5%of the controls and 97.2%of the CHC patients,whereas the SNP rs8099917 genotyping was successful in 95.5%of the controls and 100%of the CHC patients.The genotype and allele distributions for both rs12979860 and rs8099917 were significantly different between the control and CHC patient groups,with significantly higher genotype frequencies of CC and TT in the controls(P=0.037 and 0.046,respectively)and of TT and GG in the CHC patients(P=0.0009and 0.0001,respectively).Analysis of the CHC patients who achieved sustained virological response(SVR)to treatment(n=55)indicated that the rs12979860 C allele and CC genotype were predictors of SVR(P=0.02).No significant correlation was found between rs8099917 genotypes and treatment response,but carriers of the T allele showed significantly higher rates of SVR(P=0.02).Linkage disequilibrium analysis of the group that achieved SVR showed a significant association between rs12979860 and rs8099917(P=0.07).CONCLUSION:The higher allele frequency of rs12979860 C and rs8099917 T observed in non-HCVinfected individuals may indicate a potential pro
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关 键 词: | Hepatitis C Interleukin 28B Single nucleotide polymorphisms Sustained virological response Brazil |
收稿时间: | 2013 Jan 21 |
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