| 一氧化氮合酶在大鼠大肠癌细胞中的表达及意义 |
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| 引用本文： | 詹江华. 一氧化氮合酶在大鼠大肠癌细胞中的表达及意义[J]. 中华普通外科杂志, 2003, 18(11): 666-667 |
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| 作者姓名： | 詹江华 |
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| 作者单位： | 300074,天津儿童医院外科 |
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| 摘 要： | 目的研究一氧化氮合酶(NOS)在大鼠大肠癌发生中的表达情况。方法应用免疫组化技术检测1，2二甲基肼(1，2-DMH)诱导的Wistat大鼠大肠癌模型中内皮型NOS(eNOS)，神经元型NOS(nNOS)和诱生型NOS(iNOS)在正常黏膜和大肠癌中的表达情况。结果eNOS和nNOS在正常黏膜中表达，在大肠癌中不表达；iNOS在正常黏膜中表达率很低(10%)，在大肠癌中高表达(87％)。结论在1，2-DMH诱导的大肠癌模型中，iNOS表达与大肠癌的发生关系密切。
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| 关 键 词： | 一氧化氮合酶 大鼠 大肠癌 表达 癌细胞 免疫组化 二甲基肼 |
| 修稿时间： | 2003-01-14 |
Expression of NOS in the rat colorectal carcinoma cells |
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| ZHAN Jiang-hua. Expression of NOS in the rat colorectal carcinoma cells[J]. Chinese Journal of General Surgery, 2003, 18(11): 666-667 |
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| Authors: | ZHAN Jiang-hua |
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| Affiliation: | ZHAN Jiang-hua. Department of Pediatric Surgery,Tianjin Children's Hospital,Tianjin 300074,China |
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| Abstract: | Objective To study the expression of nitric oxide synthase (NOS) during the development of rat colorectal carcinoma. Methods Rat colon carcinoma was induced by injection of 1,2- dimethylhydrazine (1,2-DMH).The expression of eNOS, nNOS and iNOS in normal colon mucosa and colorectal carcinoma was observed by immunohistochemistry. ResultseNOS and nNOS were expressed in normal mucosa. Neither eNOS nor nNOS alterations were observed in carcinoma tissues,iNOS expression was very strong in carcinoma samples(87%)and only 10% in normal colon mucosa. ConclusionOur results show that iNOS may play an important role in the 1,2-DMH induced colon tumorigenesis. |
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| Keywords: | Colorectal neoplasms Nitric oxide synthase |
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