不同运动训练方式和补剂对大鼠肌糖原生物合成的影响

以成年雄性SD大鼠为研究对象 ,采用正交设计法安排实验 ,研究了耐力训练、间歇高强度训练、肌酸、谷氨酰胺四因素对耗竭运动后恢复期及正常训练后安静状态下大鼠肌糖原合成量的影响 ,结果显示 :耐力训练和间歇高强度训练大鼠在耗竭运动后 2 4小时 ,前糖原 (PG)、大糖原(MG)以及总糖原的累计合成量均显著高于未训练大鼠 (P <0 0 5 ) ,补充谷氨酰胺可使正常训练大鼠安静状态下肌糖原水平显著增高 (P <0 0 5 ) ,补肌酸对肌糖原合成量未见明显影响 (P >0 0 5 )。同时 ,耐力训练与间歇高强度训练的交互作用也使肌糖原合成量显著增加 (P <0 0 5 )。

Effects of Exercise Training Styles and Sport Supplements on Muscle Glycogen Biosynthesis in Rats

Adult male SD rats were used as research subject, orthoganal design method was adopted to arrange the experimental procedure, endurance swim training, high-intensity intermittent training, creatine and glutamine were selected as the studying factors and their effects on muscle glycogen accumulation were observed. After 2 weeks of exercise training and tonic supplementing, rats were made to perform a glycogen-depletion exercise, muscle proglycogen (PG)and macroglycogen(MG) content were tested at different time point during recovery period. Some rats were scheduled rest after training (do no glycogen-depletion exercise) till to be killed. The result shows that both endurance exercise training and high-intensity intermittent exercise training rats obtain a more higher muscle PG, MG and total glycogen accumulation than that of sedentary rats 24 hours after depletion exercise (P <0 05). Glutamine supplementation significantly increases skeletal muscle glycogen content of rats at rest status after training (P < 0 05), while creatine has no remarkable effect on glycogen accumulation (P >0 05). The interaction between endurance exercise training and high-intensity intermittent exercise training is also capable of increasing muscle glycogen resynthesis significantly ( P <0 05).