青藏高原喜马拉雅旱獭疫源地鼠疫菌质粒种类及特性的研究

用琼脂糖凝胶电泳的方法，研究了青藏高原喜马拉旱獭鼠疫自然疫源地的９１４株鼠疫菌，共观察到１０种质粒，其中大质粒分子量差异较大，为我国各疫源地中鼠疫菌所含大质粒最独特的地区，其分布特征是：含５２×１０＾５质粒的菌株分布在祁连山南、北木刻级青海湖的环湖地区；带６５×１０＾６闰的菌，主要见于青南高原。当金山口甘南山地、冈底斯山与喜马拉雅山之间和藏南谷地以及新疆南部亦有零星分布。鉴于我国青藏高原喜马拉雅旱

Study on Characterization and Category of Yersinia pestis Plasmids in Marmota himalayana Plague Foci in Qinghai-Tibet Plateau

There were 10 kinds of plasmids being found by using the method of Agrose Electrophoresisfrom 914 strains of Yersinia pestis collected from the plague natural foci of Marmota himalayana in Qinghai-Tibet plateau. The molecular weights of large plasmids from this region were varied in comparing with thatfrom other foci in China, The strains harboured 52 MD plasmid distributed within the areas of south andnorth Qilianshan mountain and the area around Qinghai lake. The 65MD plasmid dispersed in the southernpart of Qinghai,the mountain pass of Dangjingshan,the mountain area of southern part of Gansu province,thevalley of southern part of Tibet,the area between Gangdisishan and Himalayashan mountdin,and the area ofHotan and Ruoqiang of southern Xinjiang.The strains with 92 MD plasmid spread among Tanggula areasaround Naqu district in northern part of Tibet,and scattered in the southern part of Qinghai plateau.The dis-tribution specifities,the varieties of molecular weight of the large plasmids indicated the complexity and diver-sity of space structure in this foci,which possessed signifficances in term of epidemiology.