谷氨酰胺心肌缺血再灌注损伤治疗中的效果与机制分析

目的：探讨谷氨酰胺心肌缺血再灌注损伤治疗中的效果与机制。方法：研究时间为2015年2月到2015年10月,选择清洁级SPF大鼠48只完全随机分为包括假手术组、缺血再灌注组和谷氨酰胺组,每组16只,建立心肌缺血再灌注损伤后,谷氨酰胺组给予腹腔注射谷氨酰胺治疗,观察三组心功能与炎症因子的表达变化情况。结果：缺血再灌注组和谷氨酰胺组都顺利建立心肌缺血再灌注损伤模型,表现为抬高的ST段逐步恢复,MAP逐步恢复,左心室颜色转红。同时心功能无明显变化,提示谷氨酰胺对心脏的无明显毒性。三组心率HR在缺血前无明显差异,但缺血再灌注组再灌注后有下降趋势,而再灌注30min后谷氨酰胺组HR较缺血再灌注组升高(P<0.05)。假手术组给药前后MAP无明显变化,缺血再灌注组与谷氨酰胺组在给药后出现轻度下降,不过对比差异无统计学意义(P>0.05)。谷氨酰胺组再灌30min的TNF-α与IL-6含量均明显低于缺血再灌注组(P<0.05),而与假手术组比较差异均无统计学意义(P>0.05)。结论：谷氨酰胺可以对抗缺血再灌注损伤引起的心肌损伤,发挥心脏保护作用,其机制可能与抑制炎症反应有关。

The effects and mechanism of glutamine in the treatment of myocardial ischemia reperfusion injury

Objective: To investigate the effects and mechanism of glutamine in the treatment of myocardial ischemia and reperfusion injury. Methods: The study time were from February 2015 to October 2015, 48 SPF clean grade Rats were completely randomized the NC group(n = 16), IR group(n = 16) and Glu group (n = 16), After were establishment of myocardial ischemia and reperfusion injury, the Glu group were received intraperitoneal injection of glutamyl amine treatment. observed the expression changes of the three groups of cardiac function and inflammatory factors. Results:The myocardial ischemia reperfusion injury model were successfully established in the IR group and Glu group. There were no significant difference in HR compared among the three groups before and after ischemia, but the IR group were showed decreasing trend after reperfusion, and the HR in the Glu group were higher than that in the IR group after reperfusion in reperfusion 30min (P<0.05). There were no significant change in MAP before and after administration compared among the three groups, and there were a slight decrease in the IR group and Glu group after administration, but there were no significant difference compared between the groups (P>0.05). The TNF- and IL-6 levels in the reperfusion 30min in the Glu group were significantly lower than that in IR group (P<0.05), but there was no significant difference compared between the Glu group and the NC group(P>0.05). Conclusion: Glutamine can protect against myocardial injury induced by ischemia and reperfusion injury, and its mechanism may be related to the inhibition of inflammatory reaction.