| 痢疾杆菌和大肠杆菌粘附和侵入HeLa细胞的观察 |
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| 引用本文： | 郭庆福,刘一平,周佳敏,高杰英.痢疾杆菌和大肠杆菌粘附和侵入HeLa细胞的观察[J].军事医学科学院院刊,1987(5). |
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| 作者姓名： | 郭庆福 刘一平 周佳敏 高杰英 |
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| 作者单位： | 军事医学科学院微生物流行病研究所
(郭庆福，刘一平，周佳敏)，军事医学科学院微生物流行病研究所(高杰英) |
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| 摘 要： | 本文用镜检法和活菌计数法观察了痢疾杆菌对HeLa细胞粘附、侵袭和在胞内繁殖的感染情况,测定了不同株痢疾杆菌、大肠杆菌及杂交菌株的毒力。
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| 关 键 词： | HeLa细胞 粘附力 侵袭力 痢疾杆菌 大肠杆菌 |
OBSERVATIONS ON THE ADESIVE AND PENETRATING ABILITIES OF Shigella spp. AND E. coli TO HeLa CELLS |
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| Guo Qingfu Liu Yiping Zhou Jiamin Gao Jieying.OBSERVATIONS ON THE ADESIVE AND PENETRATING ABILITIES OF Shigella spp. AND E. coli TO HeLa CELLS[J].Bulletin of the Academy of Military Medical Sciences,1987(5). |
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| Authors: | Guo Qingfu Liu Yiping Zhou Jiamin Gao Jieying |
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| Abstract: | Two kinds of HeLa cell infectivity assay to test the virulence of ShigeUa spp. and E. coli were established. One was counting the percentage of infected HeLa cell on the Giemsa stained cover slides under optical microscope, and the other, counting the numbers of bacteria adhere to and penetrate into the HeLa cells. The processes of adhesion and invasion of Shigella flexneri 2a into the HeLa cere were observed by the scanning and transmission microscopies. The adhesive and penetrating abilities of the virulent and avirulent strains of S. flexneri, the smooth ancl its rough mutant of S. sonnei, the hybrid of S. sonnei and Salmonella typhi Ty21a strains were assayed. The differences of the ability of several strains E.coli, with or without colonization factors, to adhere to the HeLa cells monolayers were significant. |
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| Keywords: | HeLa cell adhesion penetration Shigella Spp E coli |
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