中药蜂房对口腔常驻菌形成人工致龋生物膜影响的实验研究

目的　研究蜂房对口腔常驻菌形成人工致龋生物膜的影响,探讨其防龋应用前景。方法　在体外模型中的羟磷灰石片上定植4种口腔常驻菌,以形成实验性生物膜。实验组定时给予含4 g/L蜂房提取物的蔗糖溶液处理生物膜,观察其pH的动态变化,以及生物膜表面微结构与微生物组成的改变。以同浓度的蔗糖溶液与蒸馏水分别作为阳性与阴性对照。结果　①实验组培养室内pH值呈先下降,再逐步恢复正常的波浪形变化。②蜂房的加入能抑制变链对羟磷灰石片的黏附。③蜂房的加入可使生物膜表面胞外基质的量减少。结论　蜂房作为一种潜在的防龋药物可能有着良好的应用前景。

Evaluation of the Cario-static Effect of Nidus vespae on Biofilm Model in vitro

OBJECTIVE: To evaluate the Nidus vespae's cario-static effect on biofilm model in vitro. METHODS: A four-organism bacterial consortium was grown in a biofilm model on hydroxyapatite (HA) discs in a continuous culture system and exposed to repeated solution pulsing respectively. There were three parallel-connected flow cells in the model, so were the three groups with different solution pulsed in. Negative control group was pulsed with distilled water, positive control group was pulsed with 250 mmol/L sucrose solution as well. While 4.0 g/L Nidus vespae together with 250 mmol/L sucrose solution was pulsed in the experiment group. During the experiment, the pH responses against the pulses were recorded. After the 6 pulses, the biofilm surface structure was observed with a scan electron microscope and the population on the biofilm was enumerated. RESULTS: Nidus vespae can significantly inhibit the adherence of Streptococcus mutans to HA discs compared with the control group of 250 mmol/L sucrose pulsed in, and can facilitate the remove of acid products. It is also found that the extra-cellular polysaccharide is reduced with the pulsing of Nidus vespae. CONCLUSION: Nidus vespae in the biofilm model can partially decrease the cariogenic response of sucrose solution pulsed in.