| 反相高效液相色谱结合柱前衍生化法测定人血清中L-门冬酰胺 |
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| 引用本文： | 杨志晖,王清清,王秀中,欧伦,宋海峰.反相高效液相色谱结合柱前衍生化法测定人血清中L-门冬酰胺[J].药物分析杂志,2011(12). |
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| 作者姓名： | 杨志晖 王清清 王秀中 欧伦 宋海峰 |
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| 作者单位： | 军事医学科学院放射与辐射医学研究所药理毒理研究室;空军总医院药学部; |
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| 摘 要： | 目的:建立反相高效液相色谱法(RP-HPLC)快速准确测定人血清中L-门冬酰胺(L-ASN)。方法:用已耗竭L-ASN的人血清为空白生物基质建立标准曲线,邻苯二甲醛在线柱前衍生样品,ZORBAX Eclipse AAA柱(4.6 mm×150 mm,5μm)分离衍生化产物。采用内标法根据荧光响应值定量分析。结果:L-ASN与内标高丝氨酸的衍生物均在16 min内出峰,在2～100μmol.L-1的范围内方法线性关系良好(r>0.999),最低定量限为2μmol.L-1,日内与日间精密度(RSD)均小于12.9%,回收率在96.9%～108.3%之间,平均提取回收率为58.2%,血清中L-ASN-80℃冻存四周保持稳定。结论:建立了RP-HPLC结合柱前衍生化测定人血清中L-ASN的方法,并应用于临床血清样品中L-ASN的定量测定。
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| 关 键 词： | 反相高效液相色谱法 血清样品 L-门冬酰胺(L-ASN) 门冬酰胺酶(ASNase) 邻苯二甲醛 柱前在线衍生 |
Determination of L-asparagine in human serum on o-phthalaldehyde derivatives by RP-HPLC with pre-column derivatization |
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| YANG Zhi-hui,WANG Qing-qing,WANG Xiu-zhong,OU Lun,SONG Hai-feng.Determination of L-asparagine in human serum on o-phthalaldehyde derivatives by RP-HPLC with pre-column derivatization[J].Chinese Journal of Pharmaceutical Analysis,2011(12). |
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| Authors: | YANG Zhi-hui WANG Qing-qing WANG Xiu-zhong OU Lun SONG Hai-feng |
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| Institution: | YANG Zhi-hui1,2,WANG Qing-qing1,WANG Xiu-zhong1,OU Lun1,SONG Hai-feng1 (1.Department of pharmacology and toxicology,Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,China,2.Department of pharmacy,Air Force General Hospital,Beijing 100142,China) |
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| Abstract: | Objective:To develop a rapid and accurate reversed phase high performance liquid chromatography(RP-HPLC) method for determination of L-asparagine(L-ASN) in human serum.Method:The calibration curves were established with a specific L-ASN-exhausted serum,which had been treated by spiked asparaginase.Amino acids derivatization with o-phthalaldehyde was performed automatically by the auto-sampler.After separated on a ZORBAX Eclipse AAA column,the o-phthalaldehyde derivatives were quantitated by internal standar... |
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| Keywords: | RP-HPLC serum L-ASN asparaginase o-phthalaldehyde(OPA) derivatization automatically |
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