| 早老性痴呆转基因小鼠的研究 |
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| 引用本文： | 秦川,常洋,朱华,尹红星,高虹,刘亚莉. 早老性痴呆转基因小鼠的研究[J]. 中国老年学杂志, 2000, 20(1): 31-32 |
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| 作者姓名： | 秦川 常洋 朱华 尹红星 高虹 刘亚莉 |
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| 作者单位： | 中国医学科学院中国协和医科大学实验动物研究所,北京,100021 |
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| 摘 要： | 目的 建立早老性痴呆的转基因小鼠，为进一步的发病机理研究及药物筛选提供动物模型。方法 显微注射方法制备转基因小鼠，通过ＰＣＲ、Ｓｏｕｔｈｅｒｎ杂交鉴定。结果 质粒ｐＰｄＡＰ７５１的Ｔｔｈ１１１Ⅰ＋ＸｂａⅠ双酶切片段长度为４．３ｋｂ，经回收、纯化后，注射到小鼠受精卵的雄性原核，经ＰＣＲ，Ｓｏｕｔｈｅｒｎ杂交鉴定，确定首建鼠一只。首建鼠现已传代建系。结论 通过显微注射的方法，建立了早老性痴呆的转基因动
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| 关 键 词： | 早老性痴呆 APP 转基因 动物模型 |
| 修稿时间： | 1999-01-14 |
Study of the Transgenic mice model of Alzheimer disease by use of Human Mutant APP gene |
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| Qin Chuan,Chang Yang,Zhu Hua et al Institute of Laboratory Animal Science,Chinese Academy of Medical Science. Study of the Transgenic mice model of Alzheimer disease by use of Human Mutant APP gene[J]. Chinese Journal of Gerontology, 2000, 20(1): 31-32 |
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| Authors: | Qin Chuan Chang Yang Zhu Hua et al Institute of Laboratory Animal Science Chinese Academy of Medical Science |
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| Affiliation: | Qin Chuan,Chang Yang,Zhu Hua et al Institute of Laboratory Animal Science,Chinese Academy of Medical Science. Beijing 100021 |
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| Abstract: | For the etiopathological studies and drug development Alzheimer disease(AD), the transgenic mice of AD were used. Methods Obtain the transgenic mice by microinjection, and confirmed by polymerase chair reaction(PCR) and Southern blot.Results The transgene was isolated by Tth111I XbaI from the recombinant pPDAP751, and purified by equilibrium centrifugation in CsC1 gradient. Transgene was microinjected into the male pronucleus of the zygotes. The founder was tested by PCR and Southern blot.Conclusions By microinjection, the transgenic mice of AD were establish, which carry the human mutant APP cDNA751. |
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| Keywords: | animal model of Alzheimer disease APP transgene |
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