辛伐他汀对心肌梗死后大鼠左室重构及心肌β3整合素表达的影响

目的研究辛伐他汀对心肌梗死后4wk雄性SD大鼠心功能、左室质量指数、心肌组织β3整合素及Ⅰ型胶原表达的影响。方法冠脉结扎后24h存活的大鼠随机分2组:辛伐他汀组(辛伐他汀30mg·kg~(-1)·d~(-1)灌胃,n=14)和模型组(等量生理盐水灌胃,n=14)。另设假手术组(Sham组,等量生理盐水灌胃,n=9),治疗4wk。结果模型组左室质量指数(2.53±s 0.14)mg·g~(-1)及左室舒张末压(13.9±1.3)mmHg均高于Sham组(P<0.01);辛伐他汀组分别为(2.24±0.08)mg·g~(-1)和(5.6±3.6)mmHg,低于模型组(P<0.01)。模型组胶原容积分数为(12.6±3_3)%,较Sham组增加(P<0.01);辛伐他汀组为(5.6±0.5)%,低于模型组(P<0.01)。模型组β3整合素和Ⅰ型胶原表达较Sham组增多(P<0.01),而辛伐他汀组低于模型组(P<0.01)。结论辛伐他汀可改善心肌梗死后心功能和左室重构,其机制之一可能是抑制β3整合素表达。

Effects of simvastatin on left ventricular remodeling and expression of β3integrin after acute myocardial infarction in rats

AIM To study the impact of simvastatin(Sim)on left ventricular remodeling,cardiac function,expression ofβ3 integrins and collagenⅠ4 wk after acute myocardial infarction(AMI)in male SD rats.METHODS Twenty-four hours after ligating left coronary artery,the survived rats were randomly assigned to:Sire group(Sim 30 mg·kg~(-1)·d~(-1),gastric infusion,n=14)and model(myocardial infarction,MI)group (equal volume of saline for gastric infusion,n=14).In addition,Sham-operated group(n=9)was selected as the non-infarction control.After 4 wk of therapy,cardiac function,hemodynamics,ventricular weight/body weight ratio,non-infarcted myocardial collagen volume fraction(CVF),expression ofβ3 integrins and collagenⅠwere investigated.RESULTS In model group,ventricular weight/body weight ratio,left ventricular end diastolic pressure(LVEDP),CVF and expression ofβ3 integrins and collagenⅠwere all significantly higher (P<0.01)than those of Sham-operated group.For Sire group,ventricular weight/body weight ratio,LVEDP, CVF,expression ofβ3 integrins and collagenⅠwere all significantly lower(P<0.01)than those in model group.CONCLUSION Simvastatin can improve hemodynamics and left ventricle function due to its inhibition of 133 integrins probably as one of the crucial mechanisms.