Musashi-2在急性髓系白血病干细胞中的表达研究

本研究旨在探讨检测AML患者Musashi-2（Msi2）的表达与其他临床参数特别是CD34表达的相关性.提取临床初诊确诊为急性髓系白血病（AML）患者骨髓标本的总RNA,荧光定量RT-PCR检测患者Msi2的表达水平;流式细胞术检测上述病人的CD34表达水平;综合分析其他实验室检查资料和患者的临床表现,分析初诊AML患者Msi2表达情况以及Msi2表达与CD34表达的相关性.结果表明：①初诊AML患者骨髓Msi2表达显著高于正常对照骨髓（P＜0.05）,各亚型中Msi2表达以M1、M4和M5中表达较高,显著高于AML其他亚型中的表达（P＜0.05）;②Msi2的表达与患者年龄、性别、初诊时外周血白细胞数、AML1/ ETO融合基因、PML/ RARa融合基因均无关（P＞0.05）;③CD34＋组AML患者Msi2表达显著高于CD34-AML患者（P＜0.05）.结论：Msi2表达于白血病干细胞,Msi2在初诊AML中高表达,以M1、M4和M5亚型中表达尤为显著,在CD34＋ AML患者中Msi2高表达.

Expression of Musashi-2 Gene in Leukemia Stem Cells from Acute Myeloid Leukemia Patients

This study was aimed to detect the expression of Musashi-2 （Msi2） in acute myeloid leukemia（AML） and investigate the relationship between Msi2 and other clinical parameters,especially CD34.A total RNA was extracted from bone marrow of newly diagnosed AML patietns.The Msi2 mRNA expression in newly diagnosed AML patients was detected with real-time fluorescence quantitative RT-PCR.The expression level of CD34 in above-menthioned patients was detected by flow cytometry （FCM）.The relationship between the expression of Msi2 mRNA and clinical outcome in AML patients was analysed.The results showed that （1） the expression of Msi2 mRNA in newly diagnosed AML patients was much higher than that in healthy volunteers（P 〈0.05）,especially in M1,M4 and M5 patients; （2） the expression level of Msi2 did not correlate with age,sex,white blood cell count of peripheral blood,AML1/ETO and PML/RARa fusion gene （P 〉 0.05） ; （3） Msi2 expression level in patients with CD34 ＋ cells was significantly higher than that in patients with CD34-cells （P 〈 0.05）.It is concluded that the Msi2 mRNA expresses in leukamia stem cells,the high expression of Msi2 mRNA has been found in newly diagnosed AML patients,especially in M1,M4 and M5 patients,the high expression also has been observed in patients with CD34＋.