Green fluorescent protein (GFP): applications in cell-based assays for drug discovery |
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| Affiliation: | 1. Department of Chemistry, Mashhad Branch, Islamic Azad University, Mashhad, Iran;2. Research Center for Animal Development Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad 917568, Iran;1. Faculty of Chemistry, Bu-Ali Sina University, Hamadan, Iran;2. Department of Chemistry, Central Tehran Branch, Islamic Azad University, Tehran, Iran;3. Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran;4. Department of Pharmaceutics, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran;5. Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia, Katowice, Poland;1. Materials and Physics Division, School of Advanced Sciences, Vellore Institute of Technology (VIT), Chennai 600127,Tamil Nadu, India;2. Catalysis and Nanomaterials Research Laboratory, Department of Chemistry, Loyola College (Autonomous), Chennai 600034, Tamil Nadu, India;3. Department of Physics, College of Science, University of Bahrain, P.O. Box 32038, Bahrain;1. School of Chemistry and Life Science, Anshan Normal University, PO Box 114007, Anshan, People''s Republic of China;2. Liaoning Key Laboratory of Development and Utilization for Natural Products Active Molecules, Anshan Normal University, PO Box 114007, Anshan, People’s Republic of China;3. School of Materials and Metallurgy, University of Science and Technology Liaoning, PO Box 114051, Anshan, People''s Republic of China;4. Central Research Laboratory, Esfarayen University of Technology, Esfarayen, North Khorasan, Iran |
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| Abstract: | Green fluorescent protein (GFP) is a powerful tool for cell-based assays owing to the intrinsic fluorescence of this protein that allows real-time analysis of molecular events in living cells. A number of GFP variants have been developed with optimal properties for both high-throughput screening and high-content screening. The author discusses advances in basic GFP technology, including the discovery of fluorescent proteins from divergent bioluminescent species, as well as the development of various GFP biosensors suited to the drug discovery process. |
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