Trisomy 8 in acute promyelocytic leukaemia: an interphase study by fluorescence in situ hybridization |
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Authors: | Y. L. KWONG K. F. WONG T. K. CHAN |
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Affiliation: | University Department of Medicine, Queen Mary Hospital, Hong Kong;Department of Pathology, Queen Elizabeth Hospital, Hong Kong |
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Abstract: | Summary. Acute promyelocytic leukaemia (APL) is characterized by t(15;17)(q24;q21). Trisomy 8 is the commonest accompanying karyotypic aberration. We investigated 14 APL patients for trisomy 8 using fluorescence in situ hybridization (FISH). Conventional cytogenetic analysis showed trisomy 8 in two of nine successfully karyotyped cases. With FISH, a possible third case showing a subclone (1-2-5%) with trisomy 8 was found. The trisomy 8 clone size defined by karyotyping and FISH was concordant in one case and discordant in another, in which trisomy 8 was found in 100% of metaphases but only in 48% of leukaemic prbmyelocytes by FISH. Therefore trisomy 8 was mosaic in all the cases, suggesting that it had arisen from clonal evolution. AU-trans-retinoic acid successfully induced morphologic remission in both cases with trisomy 8. |
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Keywords: | trisomy 8 acute promyelocytic leukaemia fluorescence in situ hybridization |
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